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| Status |
Public on Feb 17, 2026 |
| Title |
Müller glial and microglial responses coupled to recovery of cone photoreceptors following limited cone ablation in zebrafish retina |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We treated gnat2:nfsb-mCherry transgenic adult zebrafish with 24-hour 10 mM Mtz immersion exposure, triggering transient cone stress and subsequent glial and microglial responses.
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| Overall design |
Adult gnat2:nfsb-mCherry transgenic zebrafish were exposed to 0.1% DMSO or 10 mM Mtz in 0.1% DMSO for 24 hours by immersion followed by washout to clean water. For sequencing, whole retinas were isolated, dissociated to single cell suspension, and analyzed using scRNA-seq. Samples were isolated at 96 hours post treatment (DMSO and MTZ 96 HPT) and at 120 hours post treatment (MTZ 120 HPT). Two retinas were pooled per sample for sequencing.
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| Contributor(s) |
Weimar H, Farre A, Rumford J, Mitchell D |
| Citation(s) |
41691075 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 EY030467 |
Macrophage Determinants of Retinal Regeneration |
UNIVERSITY OF IDAHO |
Diana Mitchell |
|
| BioProject |
PRJNA1304131 |
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| Submission date |
Aug 11, 2025 |
| Last update date |
Feb 18, 2026 |
| Contact name |
Sarah Hendricks |
| Organization name |
University of Idaho
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| Street address |
685 S. Line Street, IRIC 210
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| City |
Moscow |
| State/province |
ID |
| ZIP/Postal code |
83843 |
| Country |
USA |
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| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
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| Samples (3) |
| GSM9163332 |
Whole retina single cell suspension, collected at 96 hours post DMSO treatment |
| GSM9163333 |
Whole retina single cell suspension, collected at 96 hours post Mtz treatment |
| GSM9163334 |
Whole retina single cell suspension, collected at 120 hours post Mtz treatment |
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