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| Status |
Public on Sep 21, 2011 |
| Title |
Whole Genome Expression in Peripheral-Blood Samples of Workers Professionally Exposed to Polycyclic Aromatic Hydrocarbons |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
This study aims to examine global gene expression profiles before and after the work-shift among coke-oven workers (COW). COW work six consecutive days and then take two days off. Two blood and urine samples in each worker were collected before starting to work after two-days off and end-of-shift in the sixth-day work in 2009. Altered gene expressions (ratio of gene expression levels between end-of-shift and pre-shift work) were performed by Human OneArray expression system which probes ~30,000-transcription expression profiling of human genes. Sixteen workers, all men, were enrolled in this study. Median urinary 1-hydroxypyrene (1OHP) levels (umole/mole creatinine) in end-of-shift work were significantly higher than those in pre-shift work (2.58 vs. 0.29, p = 0.0002). Among the 20,341 genes which passed experimental quality control, 26 gene expression changes, 7 positively- and 19 negatively-, were highly correlated with across-the-shift urinary 1OHP levels (end-of-shift – pre-shift 1OHP) (p-value < 0.001). The high and low exposure groups of across-the-shift urinary 1OHP levels dichotomized in ~2.00 µmole/mole creatinine were able to be distinguished by these 26 genes. Some of them are known to be involved in apoptosis, chromosome stability/DNA repair, cell cycle control/tumor suppressor, cell adhesion, development/spermatogenesis, immune function, and neuronal cell function.
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| Overall design |
Sixteen coke-oven workers who had worked in one of two coke-oven plants for at least one year in the largest steel company in Taiwan voluntarily participated in this study between July-October, 2009. Coke-oven workers regularly work 6 days and take two days off. Thus, we collected their blood and urine samples at two different time points: one was during the pre-shift work on the first day after two days off, and the second one was at the end-of-shift work on the 6th work day. Information about age and smoking status was also collected before the collection of biological specimens.
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| Contributor(s) |
Wu M, Pan C, Su H, Huang J, Peng C, Wang W, Chou T, Lin M, Lin W, Huang C, Lee T, Ho C |
| Citation(s) |
21854004 |
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| Submission date |
Jul 08, 2011 |
| Last update date |
Aug 28, 2014 |
| Contact name |
Tzu Chi Lee |
| E-mail(s) |
charles@kmu.edu.tw
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| Organization name |
Kaohsiung Medical University
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| Department |
Department of Public Health
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| Street address |
Shihcyuan 1st Rd.
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| City |
Kaohsiung |
| ZIP/Postal code |
80708 |
| Country |
Taiwan |
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| Platforms (1) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA143317 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30504_RAW.tar |
206.7 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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