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Series GSE30495 Query DataSets for GSE30495
Status Public on Feb 21, 2012
Title Detailed transcriptomics analysis of the effect of dietary fatty acids on gene regulation in the murine heart.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Fatty acids comprise the primary energy source for the heart and are mainly taken up via hydrolysis of circulating triglyceride-rich lipoproteins. While most of the fatty acids entering the cardiomyocyte are oxidized, a small portion is involved in altering gene transcription to modulate cardiometabolic functions. So far, no in vivo model has been developed enabling study of the transcriptional effects of specific fatty acids in the intact heart. In the present study, mice were given a single oral dose of synthetic triglycerides composed of one single fatty acid. Hearts were collected 6h thereafter and used for whole genome gene expression profiling. Experiments were conducted in wild-type and PPARα−/− mice to allow exploration of the specific contribution of PPARα. It was found that: 1) linolenic acid (C18:3) had the most pronounced effect on cardiac gene expression. 2) The largest similarity in gene regulation was observed between linoleic acid (C18:2) and C18:3. Large similarity was also observed between the synthetic PPARα agonist Wy14643 and docosahexaenoic acid (C22:6). 3) Many genes were regulated by one particular treatment only. Genes regulated by one particular treatment showed large functional divergence. 4) The majority of genes responding to fatty acid treatment were regulated in a PPARα-dependent manner, emphasizing the importance of PPARα in mediating transcriptional regulation by fatty acids in the heart. 5) Several genes were robustly regulated by all or many of the fatty acids studied, mostly representing well-described targets of PPARs (e.g. Acot1, Angptl4, Ucp3). 6) Deletion and activation of PPARα had a major effect on expression of numerous genes involved in metabolism and immunity. Our analysis demonstrates the marked impact of dietary fatty acids on gene regulation in the heart via PPARα.
 
Overall design To study the transcriptional effects of specific fatty acids in the intact heart, wild type and PPARα−/− mice were given a single oral dose of 4 synthetic triglycerides composed of one single fatty acid, as well as of the synthetic PPARα agonist Wy14,643. Hearts were collected 6h after gavag and used for whole genome gene expression profiling.
 
Contributor(s) Georgiadi A, Boekschoten MV, Muller M, Kersten S
Citation(s) 22274564
Submission date Jul 07, 2011
Last update date Feb 11, 2019
Contact name Guido Hooiveld
E-mail guido.hooiveld@wur.nl
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (55)
GSM756426 WT_Control_rep1
GSM756427 WT_Control_rep2
GSM756428 WT_Control_rep3
This SubSeries is part of SuperSeries:
GSE30649 Detailed transcriptomics analysis of the effect of dietary fatty acids on gene regulation in the murine heart [superseries]
Relations
BioProject PRJNA154763

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30495_RAW.tar 189.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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