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| Status |
Public on Aug 01, 2011 |
| Title |
Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We are interested in examining the transcriptional hierarchies that are required for proper formation and function of the carpel margin meristem in Arabidopsis thaliana. The carpel margin meristem (CMM) gives rise to the ovule primorida (which begin to form around flower stage 8). We know that the loss of the seuss-3 (seu-3) and aintegumenta-1(ant-1) separately leads to a mild disruption of ovule formation, while the loss of both causes a complete loss of ovule formation. In this study we wanted to examine expression levels changed between each single mutant and the double mutant. We dissected out stage 8-10 carpel of each of the four genotypes (seu-3, ant-1, Col-0, seu-3 ant-1) pooling 15-25 carpels into one biological replicate. Then using 16 ATH1 arrays we hybridized, amplified and labeled cDNA to 16 arrays (4 replicates for each genotype). We then analyzed our data to reveal transcripts that went down in the double compared to either single mutant, and transcripts that went down more than additively in the double mutant compared to added level of the decrease of the single mutant levels.
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| Overall design |
16 arrays: 4 seuss-3, 4 aintegumenta-1, 4 Col-0, 4 seuss-3 aintegumenta-1. Each biological replicate was a sample of 15-25 pooled stage 8-10 carpels manually dissected by hand.
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| Contributor(s) |
Wynn AN, Franks RG |
| Citation(s) |
22031826 |
| Submission date |
Jul 07, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
April N Wynn |
| E-mail(s) |
anwynn2@ncsu.edu
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| Phone |
919-513-0462
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| Organization name |
NC State University
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| Department |
Genetics
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| Lab |
Franks
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| Street address |
Campus Box 7614
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| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695-7614 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (16)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA143511 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30492_RAW.tar |
35.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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