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Series GSE30329 Query DataSets for GSE30329
Status Public on Jan 01, 2016
Title Global gene expression analysis of Yersinia pestis Ysp AHL quorum sensing at 30°C
Platform organism Yersinia pestis CO92
Sample organism Yersinia pestis
Experiment type Expression profiling by array
Summary Quorum sensing is a cell to cell communication process that involves chemical signaling. Yersinia pestis, the agent of plague, has two functional AHL quorum sensing systems Ysp and Ype. For several reasons, it was not clear what effect AHL pathways have on virulence gene expression and survival in the two different hosts, flea and human. To investigate to what effect Ysp AHL quorum sensing has on gene expression, we conducted microarray studies comparing Yersinia pestis CO92 (∆pgm) to a single AHL mutant strain (∆pgm ΔyspI) at 30°C.
 
Overall design Six independent RNA samples from Y. pestis CO92 Δpgm ΔyspI cultures were paired with six independent RNA samples from control Y. pestis CO92 R88 cultures for hybridization to six two-color microarrays. For three arrays, the control RNA sample was labeled with Alexa 555 dye and the experimental RNA sample was labeled with Alexa 647 dye; the dyes were reversed for the other three arrays to account for any dye bias.
 
Contributor(s) Minion C, Yu J
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Submission date Jun 30, 2011
Last update date Jan 01, 2016
Contact name Chris Minion
E-mail(s) fcminion@iastate.edu
Phone 515-294-6347
Fax 515-294-8500
Organization name Iowa State University
Department VMPM
Street address 1130 Vet Med
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL9009 JCVI PFGRC Yersinia pestis 18K v5 array designed primarily based on strain CO92
Samples (6)
GSM752008 Δpgm vs. Δpgm ΔyspI at 30C rep1
GSM752009 Δpgm vs. Δpgm ΔyspI at 30C rep2
GSM752010 Δpgm vs. Δpgm ΔyspI at 30C rep3
This SubSeries is part of SuperSeries:
GSE30373 Global gene expression analysis of *Yersinia pestis* AHL quorum sensing
Relations
BioProject PRJNA154801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30329_RAW.tar 2.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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