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Series GSE30164 Query DataSets for GSE30164
Status Public on Jun 23, 2011
Title Transcriptome transfer provides a model for understanding the phenotype of cardiomyocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary We show that the transfer of the adult ventricular myocyte (AVM) transcriptome into either a fibroblast or an astrocyte converts the host cell into a cardiomyocyte. Transcriptome-effected cardiomyocytes (tCardiomyocytes) display morphologies, immunocytochemical properties, and expression profiles of postnatal cardiomyocytes. Cell morphology analysis shows that tCardiomyoctes are elongated and have a similar length-to-width ratio as AVMs. These global phenotypic changes occur in a time-dependent manner and confer electroexcitability to the tCardiomyocytes. tCardiomyocyte generation does not require continuous overexpression of specific transcription factors; for example, the expression level of transcription factor Mef2c is higher in tCardiomyocytes than in fibroblasts, but similar in tCardiomyocytes and AVMs. These data highlight the dominant role of the gene expression profile in developing and maintaining cellular phenotype. The transcriptomeinduced phenotype remodeling–generated tCardiomyocyte has significant implications for understanding and modulating cardiac disease development.
Overall design Transcriptome transfer can change one cell type to another cell type. We transfect cardiomyocyte transciptome into fibroblast and astrocyte. Transcriptomes from single cells are isolated and amplified and then hybridize on Affymetrix array.
Contributor(s) Kim TK, Eberwine JH
Citation(s) 21730152
Submission date Jun 22, 2011
Last update date Feb 11, 2019
Contact name Tae Kyung Kim
Phone 215-898-0420
Fax 215-573-2236
Organization name University of Pennsylvania
Department Pharmacology
Street address 3620 Hamilton Walk
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (23)
GSM747040 fibroTIPeR, rep1
GSM747041 fibroTIPeR, rep2
GSM747042 fibroTIPeR, rep3
BioProject PRJNA143813

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Supplementary file Size Download File type/resource
GSE30164_RAW.tar 66.7 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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