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Series GSE30140 Query DataSets for GSE30140
Status Public on Dec 29, 2011
Title Expression data from livers of F2 mice (C57BL/6 X DBA/2) deficient in leptin receptor (db/db)
Organism Mus musculus
Experiment type Expression profiling by array
Summary In several models of obesity-induced diabetes, increased lipid accumulation in the liver has been associated with decreased diabetes susceptibility. For instance, deficiency in leptin receptor (db/db) leads to hyperphagia and obesity in both C57BL/6 and C57BLKS mice but, only on the C57BLKS background do the mice develop beta-cell loss leading to severe diabetes while C57BL/6 mice are relatively resistant. Liver triglyceride levels in the resistant C57BL/6 mice are 3 to 4 fold higher than in C57BLKS.
To better understand the mechanisms contributing to metabolic dysfunction in obesity-induced diabetes, we used microarrays to comprehensively profile gene expression livers of F2 mice (B57BL/6 X DBA/2) deficient in leptin receptor (db/db)
Overall design DBA/2J females were mated to C57BL/6 males carrying leptin receptor deficiency (db/+) and, F1 (db/+) offspring were interbred to produce F2 mice. Offspring deficient in leptin receptor (db/db) were fed on a chow diet until 5 weeks or 12 weeks of age and then euthanized for collection of liver tissue for RNA profiling along with other diabetes-related phenotypes.
Contributor(s) Davis RC, Lusis AJ
Citation(s) 22010005
Submission date Jun 22, 2011
Last update date Feb 11, 2019
Contact name Calvin Pan
Organization name UCLA
Street address 675 Charles Young Dr South
City Los Angeles
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (435)
GSM746117 Liver from db/db F2 mouse at 5 weeks of age [5]
GSM746118 Liver from db/db F2 mouse at 5 weeks of age [6]
GSM746119 Liver from db/db F2 mouse at 5 weeks of age [7]
BioProject PRJNA143841

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Supplementary file Size Download File type/resource
GSE30140_RAW.tar 1.5 Gb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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