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Series GSE29808 Query DataSets for GSE29808
Status Public on Oct 20, 2011
Title Oncogenic ETS proteins replace activated Ras/MAPK signaling in prostate cells.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Approximately 50% of prostate cancers have chromosomal translocations resulting in the over-expression one of four ETS family transcription factors. However, it is not known why these four four family members are selected for oncogenic roles, while other ETS proteins are not. We found that the four oncogenic ETS family members have a specific role in prostate cell migration. Using chromatin immunoprecipitation coupled with next-generation sequencing, this specific biological function was matched to a specific set of genomic targets highlighted by the presence of an AP-1 binding site. ETS/AP-1 binding sites are prototypical Ras-responsive elements, but oncogenic ETS proteins could activate a Ras/MAPK transcriptional program in the absence of MAPK activation. These findings indicate that the specific function of ETS proteins over-expressed in prostate cancer is the activation of a Ras/MAPK gene expression program in the absence of signaling pathway mutations.
Overall design ChIP sequencing two transcription factors in PC3 cells, four transcription factors plus a FLAG control in RWPE-1 cells and input DNA sequencing from each cell line.
Contributor(s) Hollenhorst PC, Graves BJ
Citation(s) 22012618
Submission date Jun 08, 2011
Last update date May 15, 2019
Contact name Peter Hollenhorst
Organization name Indiana University
Street address 1001 E Third St, Jordan Hall 104
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (9)
GSM738816 PC3 Input
GSM738817 PC3 ETV4
GSM738818 PC3 JUND
SRA SRP007519
BioProject PRJNA140921

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29808_RAW.tar 270.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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