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Series GSE29766 Query DataSets for GSE29766
Status Public on Jul 15, 2011
Title Developmental profiling of spiral ganglion neurons reveals insights into auditory circuit assembly
Organism Mus musculus
Experiment type Expression profiling by array
Summary The sense of hearing depends on the faithful transmission of sound information from the ear to the brain by spiral ganglion (SG) neurons. However, how SG neurons develop the connections and properties that underlie auditory processing is largely unknown.
We catalogued gene expression in mouse SG neurons at six developmental stages, ranging from embryonic day 12 (E12), when SG neurons first extend projections, up until postnatal day 15 (P15), after the onset of hearing. For comparison, we also analyzed the closely-related vestibular ganglion (VG) at the same time points.
Overall design To identify genes involved in SG axon guidance and branching, target selection, synaptogenesis, synaptic refinement, and synaptic function, we collected SG at E12 and E13, E16, P0, P6, and P15. We also collected VG at the same time points. For E12 and E13 time points, SG and VG were microdissected from Rnx-cre; Z/EG embryos, which express GFP in the VG. E16-P15 VG was also isolated by microdissection from Rnx-cre; Z/EG animals. E16-P15 SG neurons were isolated by FACS sorting dissociated cochlea from Mafb-GFP animals.
Contributor(s) Lu C, Goodrich L
Citation(s) 21795542
Submission date Jun 06, 2011
Last update date Feb 11, 2019
Contact name Lisa Goodrich
Organization name Harvard Medical School
Department Neurobiology
Street address 220 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (40)
GSM737978 E12 spiral ganglion E12_SG_1
GSM737979 E12 spiral ganglion E12_SG_2
GSM737980 E12 spiral ganglion E12_SG_3
BioProject PRJNA141033

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Supplementary file Size Download File type/resource
GSE29766_RAW.tar 170.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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