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Series GSE2975 Query DataSets for GSE2975
Status Public on Jul 22, 2005
Title Global analysis of Caenorhabditis elegans operons
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Caenorhabditis elegans and its relatives are unique among animals, possibly even among eukaryotes, in having operons. In these regulated multigene transcription units, a polycistronic pre-mRNA is processed to monocistronic mRNAs by 3' end formation and trans-splicing utilizing a special snRNP, the SL2 snRNP, for downstream mRNAs1. Previously, the correlation between downstream location in an operon and SL2 trans-splicing has been strong, but anecdotal. Although only 28 operons have been reported previously, the complete sequence of the genome reveals numerous gene clusters. To determine how many represent operons, we probed full-genome microarrays for SL2-containing mRNAs. We found significant enrichment for about 1200 genes including most of a group of several hundred genes represented by cDNAs that contain SL2 sequence. Analysis of their genomic arrangements indicates that >90% are downstream genes, falling in 790 distinct operons. We conclude that the genome contains at least 1000 operons, 2- 8 genes in length, that contain ~15% of C. elegans genes. Most of the operons have not been reported previously, and numerous examples of co-transcription of genes encoding functionally related proteins are evident. Inspection of the operon list should reveal heretofore unknown functional relationships.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
Overall design Computed
Web link
Contributor(s) Link C
Citation(s) 12075352
Submission date Jul 21, 2005
Last update date Mar 16, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL2653 CE_printG_H_I
GPL2655 CE_PrintJ
Samples (5)
GSM65065 mixed stage N2 (red, channel 2) vs. SL2-amplified N2 (repeat #4) (green, channel 1)
GSM65066 mixed stage N2 (red channel 2) vs. SL2-amplified mixed stage N2 (green, channel 1)
GSM65067 mixed stage N2 (red, channel 2) vs. SL2-amplified N2 (repeat #1) (green, channel 1)
BioProject PRJNA91921

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