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Series GSE2963 Query DataSets for GSE2963
Status Public on Jul 20, 2005
Title Genetic Analysis of Pathways Regulated by the von Hippel-Lindau Tumor Suppressor in Caenorhabditis elegans
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary The von Hippel-Lindau (VHL) tumor suppressor functions as a ubiquitin ligase that mediates proteolytic inactivation of hydroxylated a subunits of hypoxia-inducible factor (HIF). Although studies of VHL defective renal carcinoma cells suggest the existence of other VHL tumor suppressor pathways, dysregulation of the HIF transcriptional cascade has extensive effects that make it difficult to distinguish whether, and to what extent, observed abnormalities in these cells represent effects on pathways that are distinct from HIF. Here, we report on a genetic analysis of HIF dependent and independent effects of VHL inactivation by studying gene expression patterns in C. elegans. We show tight conservation of the HIF-1/VHL-1/EGL-9 hydroxylase pathway. However, persisting differential gene expression in hif-1 versus hif-1; vhl-1 double mutant worms clearly distinguished HIF-1 independent effects of VHL-1 inactivation. Genomic clustering, predicted functional similarities, and a common pattern of dysregulation in both vhl-1 worms and a set of mutants (dpy-18, let-268, gon-1, mig-17 and unc-6), with different defects in extracellular matrix formation, suggest that dysregulation of these genes reflects a discrete HIF-1 independent function of VHL-1 that is connected with extracellular matrix function.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Bishop T, Lau KW, Epstein AC, Kim SK, Jiang M, O'rourke D, Pugh CW, Gleadle JM, Taylor MS, Hodgkin J, Ratcliffe PJ, Genetic Analysis of Pathways Regulated by the von Hippel-Lindau Tumor Suppressor in Caenorhabditis elegans., Bishop T, et al. (2004) PLoS Biol 2(10):e289, 2004-10-01,
Keywords: Logical Set
Overall design Computed
Contributor(s) Epstein A, Bishop T
Citation(s) 15361934
Submission date Jul 19, 2005
Last update date Mar 16, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL2646 CE_printK
GPL2647 CE_printU
Samples (4)
GSM64838 wild-type (red, channel 2) vs. VHL mutant (ok161) (green, channel 1)
GSM64839 hif-1 (red, channel 2) vs.hif-1; vhl-1 (green, channel 1) repeat no. 1
GSM64840 hif-1 (red, channel 2) vs. hif-1; vhl-1 (green, channel 1) repeat no. 2
BioProject PRJNA92639

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