 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 14, 2026 |
| Title |
The bone derived from scl-progenitors micromass forms bone marrow [scRNAseq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sclerotomal progenitors derived from pluripotent stem cells hold promises for modeling skeletal development and recreating embryonic bone microenvironment to restore hematopoiesis and immunity in ageing. Current strategy to derive mouse sclerotomal progenitors suffered from low differentiation efficiency and heterogenous cell progenies. Here, we developed a fast and efficient strategy to generate sclerotomal progenitors from murine embryonic stem cells (mESCs). Naïve mESCs were differentiated through epiblast-like cells (EpiLCs) and primitive streak, and the sclerotomal fate was directly induced from primitive streak by modulating BMP and SHH signaling, achiving an 85.8% differentiation efficiency. Moreover, the resulting progenitors showed similar global gene expression profiles as primary sclerotome, possessed strong osteochondral bipotential and could recapitulate key features of endochondral ossification upon micromass-mediated osteogenic induction, including bone marrow niche regeneration. To investigate the cellular component of the bone marrow derived from scl-progenitors micromass , we performed droplet-based scRNAseq (10X Genomics) of the differentiated bone tissue.
|
| |
|
| Overall design |
Single cell RNA sequencing of the cellular component of the bone marrow derived from scl-progenitors micromass
|
| |
|
| Contributor(s) |
Deng Y, Xiong J, Li Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 14, 2025 |
| Last update date |
Apr 14, 2026 |
| Contact name |
Zhonghan Li |
| E-mail(s) |
zhonghan.li@scu.edu.cn
|
| Organization name |
Sichuan University
|
| Department |
College of Life Sciences
|
| Street address |
29 Wangjiang Road
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610064 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA1250567 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE294594_RAW.tar |
157.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
