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Series GSE29458 Query DataSets for GSE29458
Status Public on May 23, 2011
Title Expression data from PDGF driven mouse tumors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Background
Tumor heterogeneity is a major obstacle for finding effective treatment of Glioblastoma (GBM). Based on global expression analysis, GBM can be classified into distinct subtypes: Proneural, Neural, Classical and Mesenchymal. The signatures of these different tumor subtypes may reflect the phenotypes of cells giving rise to them.
However, the experimental evidence connecting any specific subtype of GBM to particular cells of origin is lacking. In addition, it is unclear how different genetic alterations interact with cells of origin in determining tumor heterogeneity. This issue cannot be addressed by studying end-stage human tumors.

Methodology/Principal Findings
To address this issue, we used retroviruses to deliver transforming genetic lesions to glial progenitors in adult mouse brain. We compared the resulting tumors to human GBM. We found that different initiating genetic lesions gave rise to tumors with different growth rates.
However all mouse tumors closely resembled the human Proneural GBM.
Comparative analysis of these mouse tumors allowed us to identify a set of genes whose expression in humans with Proneural GBM correlates with survival.

Conclusions/Significance
This study offers insights into the relationship between adult glial progenitors and Proneural GBM, and allows us to identify molecular alterations that lead to more aggressive tumor growth. In addition, we present a new preclinical model that can be used to test treatments directed at a specific type of GBM in future studies.
 
Overall design Gene expression profiling was performed on 20 tumors (12 Ptenf/f and 8 Ptenf/f; p53f/f) and 3 normal brains from mice. End stage tumors were used for expression array analysis. The platform used was Affymetrix GeneChip Mouse Genome 430A 2.0 Array. The microarray labeling, hybridization and quality controls were performed by following Affymetrix protocol.
 
Contributor(s) Lei L, Canoll P
Citation(s) 21625383, 24637229
Submission date May 23, 2011
Last update date Feb 11, 2019
Contact name Liang Lei
Organization name Columbia University
Street address 1130 St. Nicholas Ave Rm 1001
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (23)
GSM729233 Tumor in Ptenf/f mice (1pten 1)
GSM729234 Tumor in Ptenf/f mice (1pten 2)
GSM729235 Tumor in Ptenf/f mice (1pten 3)
Relations
BioProject PRJNA141577

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29458_RAW.tar 72.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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