GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE29440 Query DataSets for GSE29440
Status Public on May 24, 2011
Title Design of an improved host platform for the over expression of recombinant proteins in Escherichia coli
Organism Escherichia coli
Experiment type Expression profiling by array
Summary The objective of this work was to design an improved host platform for recombinant protein expression in E. coli. The approach involves first to create a library of the E. coli genomic DNA in different expression vectors and screen for probable transcripts which may lead to slow growing colonies and also simultaneously over-expression of recombinant proteins. To observe its effect on host performance, these genes were knocked out from the E. coli genome. A CG2 strain has been created by knocking in vhb gene gene downstream of the acetate promoter and knocking down ribB gene in DH5α and transformed with Recombinant GFP cloned in pBAD33.
Overall design E. coli DH5α (control strain), and strain CG2 were transformed with pBAD33-GFP and cultured in well controlled fed batch fermentations. The effect of three parameters was sought to be studied. The first was the effect of time post induction and thus samples were collected 0, 2, 4 and 6 hours post induction. The second parameter was the effect of host modification and hence both the control and the modified host (CG2) were studied at μ = 0.3 h-1. The third parameter was the effect of specific growth rate and thus the host (CG2) was run at μ = 0.3 h-1 and 0.6 h-1.
Contributor(s) Ghosh C, Sharma AK, Mukherjee KJ
Citation(s) 22018139
Submission date May 22, 2011
Last update date Mar 08, 2019
Contact name Krishna Jyoti Mukherjee
Phone 1126704087
Organization name New Delhi
Department School Of Biotechnology
Lab Bioprocess and Biosystems Enginerring Laboratory
Street address New Mehrauli Road
City New Delhi
State/province delhi
ZIP/Postal code 110067
Country India
Platforms (1)
GPL3154 [E_coli_2] Affymetrix E. coli Genome 2.0 Array
Samples (12)
GSM728732 Control Strain at specific growth rate 0.3h-1 0h
GSM728733 Control Strain at specific growth rate 0.3h-1 2h
GSM728734 Control Strain at specific growth rate 0.3h-1 4h
BioProject PRJNA141517

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29440_RAW.tar 12.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap