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Series GSE29194 Query DataSets for GSE29194
Status Public on Oct 07, 2011
Title Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic progenitors co-occupied with lineage specific regulators (GATA1, GATA2)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human CD34 ChIP-seq portion of this dataset.
Overall design Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008) or CEBPA (SC-9314).
Contributor(s) Trompouki E, Bowman TV, Lawton LN, Fan ZP, Wu D, DiBiase A, Martin CS, Cech JN, Sessa AK, Leblanc JL, Li P, Durand EM, Mosimann C, Heffner GC, Daley GQ, Paulson RF, Young RA, Zon LI
Citation(s) 22036566
Submission date May 10, 2011
Last update date May 15, 2019
Contact name Teresa Venezia Bowman
Organization name Children's Hospital Boston
Lab Zon Laboratory
Street address One Blackfan Circle, 7th floor
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (20)
GSM722392 GATA1_CD34eryth_bio_r1_101103_6
GSM722393 GATA1_CD34eryth_bio_r2_101103_7
GSM722394 GATA1_CD34eryth_bmp_r1_100922_4
This SubSeries is part of SuperSeries:
GSE29196 Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration
SRA SRP008214
BioProject PRJNA142953

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29194_GATA1_CD34eryth_bio_HG18_min1.0.WIG.gz 3.2 Mb (ftp)(http) WIG
GSE29194_GATA1_CD34eryth_bmp_HG18_min1.0.WIG.gz 1.8 Mb (ftp)(http) WIG
GSE29194_GATA2_CD34prog_bmp_HG18_min1.0.WIG.gz 2.2 Mb (ftp)(http) WIG
GSE29194_RAW.tar 777.4 Mb (http)(custom) TAR (of YLF)
GSE29194_SMAD1_CD34eryth_bmp_HG18_min1.0.WIG.gz 502.5 Kb (ftp)(http) WIG
GSE29194_SMAD1_CD34prog_bmp_HG18_min1.0.WIG.gz 1.1 Mb (ftp)(http) WIG
GSE29194_TCF7L2_CD34prog_bio_HG18_min1.0.WIG.gz 717.5 Kb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Processed data are available on Series record
Raw data are available in SRA

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