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| Status |
Public on May 10, 2011 |
| Title |
Transcript Profiling of Soybean Seed Compartments Using Laser Capture Microdissection Coupled With RNA-Seq |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used laser capture microdissection (LCM) to capture soybean seed compartments and profiled the transcriptomes of several compartments using next-generation sequencing. We profiled the transcriptomes of the embryo-proper and the suspensor region from globular stage embryos and the seed coat parenchyma layer of early maturation stage seeds using the Illumina GAIIx system.
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| Overall design |
Illumina sequencing of transcripts from laser-captured embryo proper and suspensor of globular stage embryo and seed coat parenchyma layer of the early maturation stage seed.
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| Contributor(s) |
Goldberg RB, Le BH, Henry KF, Kawashima T |
| Citation missing |
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| |
| Submission date |
May 09, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Bob Goldberg |
| E-mail(s) |
bobglab@mcdb.ucla.edu
|
| Phone |
310-825-3270
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| Organization name |
University of California, Los Angeles
|
| Department |
Molecular, Cell and Developmental Biology
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| Street address |
610 Charles E Young Drive East
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL11192 |
Illumina Genome Analyzer II (Glycine max) |
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| Samples (3) |
| GSM721717 |
Globular-stage embryo proper region |
| GSM721718 |
Globular-stage suspensor region |
| GSM721719 |
Early maturation-stage seed coat parenchyma region |
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| Relations |
| SRA |
SRP006766 |
| BioProject |
PRJNA140079 |
