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Series GSE29135 Query DataSets for GSE29135
Status Public on Mar 27, 2012
Title MicroRNA profiling and prediction of recurrence/relapse-free survival in stage I lung cancer
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Despite surgical treatment of stage I non-small cell lung cancer (NSCLC), one third of patients will eventually have a recurrence. Robust prognostic markers are required to better manage therapy options. MicroRNAs play important roles in human cancers. The purpose of this study is to identify miRNA expression profiles that would better predict prognosis. Small RNAs extracted from formalin-fixed and paraffin-embedded (FFPE) tumor tissues of 357 stage I NSCLC patients were profiled on the human MicroRNA expression profiling V2 panel (Illumina). The expression differences between cancer subtypes were compared by t tests. The association of miRNA expression profile with recurrence free survival (RFS) was assessed using partial Cox regression models.
Two miRNA signatures that are highly predictive of RFS were identified. The first contained 32 miRNAs derived from 357 stage I NSCLC patients independent of cancer subtype; while the second containing 27 miRNAs was adenocarcinoma specific. Both of them were validated using FFPE and/or fresh frozen tissues in independent data set with 170 stage I patients. This has important prognostic or therapeutic implications for the management of patients. The identified miRNAs hold great potential as targets for histology-specific treatment or prevention and treatment of recurrent disease.
 
Overall design Small RNAs extracted from formalin-fixed and paraffin-embedded (FFPE) tumor tissues of 357 stage I NSCLC patients were profiled on the human MicroRNA expression profiling V2 panel (Illumina).
 
Contributor(s) Lu Y, Govindan R, You M
Citation(s) 22331473
Submission date May 08, 2011
Last update date Feb 18, 2019
Contact name Yan Lu
Organization name Medical College of Wisconsin
Street address 8701 Watertown Plank Rd
City Milwaukee
ZIP/Postal code 53226
Country USA
 
Platforms (1)
GPL8179 Illumina Human v2 MicroRNA expression beadchip
Samples (387)
GSM712531 patient 101; stage IA; AD
GSM713230 patient 107; stage Ib; AD
GSM713231 patient 112; stage IA; Broncho-alveolar
Relations
BioProject PRJNA140455

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29135_RAW.tar 70.0 Kb (http)(custom) TAR
GSE29135_non-normalized_data.txt.gz 3.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data is available on Series record
Processed data included within Sample table

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