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| Status |
Public on Sep 17, 2025 |
| Title |
PLETHORAs shape Arabidopsis phyllotaxis through modulation of patterning robustness and accelerated inflorescence development [DAP-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
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| Summary |
• Phyllotaxis is the arrangement of lateral organs on a stem axis, which in Arabidopsis follows a spiral pattern. We previously described that loss of three PLETHORA (PLT) transcription factors shifts the phyllotactic spiral to novel metastable patterns, but the mechanism behind these shifts remained unclear.
• In this study, we aimed to fill this knowledge gap by performing detailed analysis of phyllotaxis in plt rosettes, inflorescences and meristems. We supplement our quantitative measurements with transcriptomic profiling of plt3 plt7 meristems, genome-wide in vitro binding assays and an EMS enhancer screen in plt3 plt5 plt7.
• Contrary to earlier beliefs, primordium positioning at the inflorescence meristem is only subtly noisier in plt3 plt5 plt7, which we attribute to loss of PLT-controlled regulation of auxin and cytokinin networks. However, the meristematic patterning defects alone cannot explain the large deviations from the spiral pattern in mature mutant inflorescences. Instead, accelerated inflorescence development of plt3 plt5 plt7 generates larger patterning deviations through joint action of meristem chirality and stem torsion. We demonstrate the importance of the relationship between chirality, torsion and internode length in tissue-twisting mutants.
• We conclude that shoot meristematic PLTs provide robustness to primordium patterning and phyllotaxis through integration of developmental processes during bolting.
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| Overall design |
PLT3, PLT5 and PLT7 DAP-seq on gDNA derived from Arabidopsis shoot apical meristems and inflorescence meristems.
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| Contributor(s) |
Kerstens M, van der Klugt F, Hofhuis H, Scheres B, Willemsen V |
| Citation(s) |
41074549 |
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| Submission date |
Feb 12, 2025 |
| Last update date |
Dec 19, 2025 |
| Contact name |
Merijn Kerstens |
| Organization name |
Wageningen University & Research
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| Department |
Plant Developmental Biology
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| Street address |
Droevendaalsesteeg 1
|
| City |
Wageningen |
| ZIP/Postal code |
6708PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA1222878 |
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