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Series GSE288261

Query DataSets for GSE288261

Status

Public on Feb 02, 2025

Title

A Novel Microglia-Specific Transcriptional Signatures Correlates with Behavioural Deficits in Neuropsychiatric Lupus (NP-SLE)

Organism

Experiment type

Expression profiling by high throughput sequencing

Summary

Systemic lupus erythematosus (SLE) can present with neuropsychiatric manifestations, collectively termed NP-SLE. This study identifies a microglia-specific transcriptional signature in mouse models of NP-SLE. Through RNA-seq analysis of microglia and brain-infiltrating macrophages, we uncover an 18-gene 'NP-SLE signature' and its correlation with behavioral deficits. We also observe enrichment of genes associated with disease-associated microglia (DAM), which have been implicated in neurodegenerative diseases. These findings suggest a critical role for microglia-intrinsic mechanisms in the onset of NP-SLE and provide new insights into the cellular and molecular pathways driving this disease.

Overall design

Bulk RNA sequencing was performed on microglia and macrophages isolated by fluorescence-activated cell sorting (FACS) from brains of wild-type (WT - Casp8flox) and Caspase-8 Removed CD11c-specific Overactive MyD88 (CReCOM - CD11cCre Casp8flox) mice at young (5-6 months) and old (11-12 months) time points.

Web link

http://10.3389/fimmu.2020.00230

Contributor(s)

Cuda C, Winter D

Citation(s)

Submission date

Jan 28, 2025

Last update date

May 04, 2025

Contact name

Deborah R Winter

E-mail(s)

deborah.winter@northwestern.edu

Phone

3125030535

Organization name

Northwestern University

Street address

240 E. Huron Street, M300

City

Chicago

State/province

IL

ZIP/Postal code

60611

Country

USA

Platforms (1)

Illumina NextSeq 500 (Mus musculus)

Samples (28)

More...

GSM8762100	Mus musculus Microglia Casp8flox/flox Female Young, Replicate 1
GSM8762101	Mus musculus Microglia Casp8flox/flox Female Young, Replicate 2
GSM8762102	Mus musculus Microglia Casp8flox/flox Female Young, Replicate 5

GSM8762103	Mus musculus Microglia Casp8flox/flox Female Young, Replicate 7
GSM8762104	Mus musculus Microglia CD11cCre Casp8flox/flox Female Young, Replicate 2
GSM8762105	Mus musculus Microglia CD11cCre Casp8flox/flox Female Young, Replicate 3
GSM8762106	Mus musculus Microglia CD11cCre Casp8flox/flox Female Young, Replicate 4
GSM8762107	Mus musculus Microglia CD11cCre Casp8flox/flox Female Young, Replicate 5
GSM8762108	Mus musculus Microglia Casp8flox/flox Female Old, Replicate 1
GSM8762109	Mus musculus Microglia Casp8flox/flox Female Old, Replicate 2
GSM8762110	Mus musculus Microglia Casp8flox/flox Female Old, Replicate 3
GSM8762111	Mus musculus Microglia Casp8flox/flox Female Old, Replicate 5
GSM8762112	Mus musculus Microglia CD11cCre Casp8flox/flox Female Old, Replicate 1
GSM8762113	Mus musculus Microglia CD11cCre Casp8flox/flox Female Old, Replicate 3
GSM8762114	Mus musculus Microglia CD11cCre Casp8flox/flox Female Old, Replicate 4
GSM8762115	Mus musculus Microglia CD11cCre Casp8flox/flox Female Old, Replicate 5
GSM8762116	Mus musculus Macrophage Casp8flox/flox Female Old, Replicate 2
GSM8762117	Mus musculus Macrophage Casp8flox/flox Female Old, Replicate 4
GSM8762118	Mus musculus Macrophage Casp8flox/flox Female Old, Replicate 7
GSM8762119	Mus musculus Macrophage Casp8flox/flox Female Young, Replicate 1
GSM8762120	Mus musculus Macrophage Casp8flox/flox Female Young, Replicate 2
GSM8762121	Mus musculus Macrophage Casp8flox/flox Female Young, Replicate 6
GSM8762122	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Old, Replicate 1
GSM8762123	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Old, Replicate 3
GSM8762124	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Old, Replicate 4
GSM8762125	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Young, Replicate 3
GSM8762126	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Young, Replicate 4
GSM8762127	Mus musculus Macrophage CD11cCre Casp8flox/flox Female Young, Replicate 5

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE288261_CReCOM_Macrophage_CPM.csv.gz	1.0 Mb	(ftp)(http)	CSV
GSE288261_CReCOM_Macrophage_counts.csv.gz	590.2 Kb	(ftp)(http)	CSV
GSE288261_CReCOM_Microglia_CPM.csv.gz	1.3 Mb	(ftp)(http)	CSV
GSE288261_CReCOM_Microglia_counts.csv.gz	666.5 Kb	(ftp)(http)	CSV
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