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Series GSE288082 Query DataSets for GSE288082
Status Public on Feb 03, 2025
Title Modeling long QT syndrome using gene-edited pigs
Organism Sus scrofa
Experiment type Expression profiling by high throughput sequencing
Summary To date, KCNH2 mutations identified in LQT2 patients have been heavily studied by heterologous expression systems, allowing for pathogenicity evaluation of a certain hERG mutation by overexpressing mutant channels. However, they fall short in mimicking the full spectrum of electrophysiological changes and ion channel remodeling that occur in cardiomyocytes under physiological conditions in the context of LQT. Due to the marked differences in the lifespan, size, anatomy and physiology from humans, current zebrafish and rodent models cannot fully mimic the abnormal QT interval diseases 2. For instance, rodents exhibit an extremely higher heart rate and a much shorter action potential duration (APD) compared to humans. IKr is the predominant repolarizing current in human ventricles while inhibition of IKr in rodents has no significant effect on ventricular repolarization, making it infeasible to study LQT2 by genetic mouse model 3. Hence, there is a crucial need to construct an animal model capable of mimicking human inherited arrhythmia conditions. To address the above scientific question, we chose to create a miniature pig model. Given many physiological similarities with humans, and breeding and genome editing advantages (when compared to non-human primates), To explore the mechanism underlying mutation of KCNH2 caused LQT, we compared the transcriptomes of KCNH2-mut pigs and WT controls
 
Overall design mRNA profiles of pig Left ventricle tissue from KCNH2-mut pigs and WT controls
 
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Submission date Jan 26, 2025
Last update date Feb 03, 2025
Contact name jiuxiao zhao
E-mail(s) 3190100251@zju.edu.cn
Phone +86 18069854372
Organization name zhejiang university
Street address Yuhangtang Road, Hangzhou 310058
City hangzhou
State/province zhejiang
ZIP/Postal code 310000
Country China
 
Platforms (1)
GPL25645 Illumina HiSeq 1500 (Sus scrofa)
Samples (8)
GSM8758756 swine-1-LV
GSM8758757 swine-2-LV
GSM8758758 swine-4-LV
Relations
BioProject PRJNA1216026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE288082_res_lqtVSwt_data.csv.gz 1.5 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA

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