|
| Status |
Public on Jan 26, 2025 |
| Title |
The T3SS structural and effector genes of Chlamydia trachomatis are expressed in distinct phenotypic cell forms. |
| Sample organism |
Chlamydia trachomatis |
| Experiment type |
Third-party reanalysis
Expression profiling by high throughput sequencing
|
| Summary |
Bacteria in the chlamydiales order are obligate intracellular parasites of eukaryotic cells. Within this order, the genus Chlamydia contains the causative agents of a number of clinically important infections of humans. Biovars of C. trachomatis are the causative agents of trachoma, the leading cause of preventable blindness worldwide, as well as sexually transmitted infections with the potential to cause pelvic inflammatory disease and infertility. Irrespective of the resulting disease, all chlamydial species share the same obligate intracellular life cycle and developmental cell forms. They are reliant on an infectious cycle consisting of at least three phenotypically distinct cell forms termed the reticulate body (RB), the intermediate body (IB) and the elementary body (EB). The EB is infectious but does not replicate. The RB replicates in the host cell but is non-infectious, while the IB is an intermediate form that transitions to the EB form. In this study, we ectopically expressed the transcriptional repressor Euo, the two nucleoid-associated proteins HctA and HctB, and the two component sensor kinase CtcB in the RB. Transcriptional analysis using RNA-seq, differential expression clustering and fluorescence in situ hybridization analysis show that the chlamydial developmental cycle is driven by three distinct regulons corresponding to the RB, IB or EB cell forms. Moreover, we show that the genes for the T3SS were cell type restricted, suggesting defined functional roles for the T3SS in specific cell forms.
|
| |
|
| Overall design |
RNA-seq analysis of Chlamydia trachomatis timeline infecting HeLA cells and ectopicaly expressing the regulatory proteins, Euo, HctA, CtcB and HctB
***************************************************************
The table below lists GEO accessions reused/reanalyzed for this study.
***************************************************************
|
| |
|
| Contributor(s) |
Scott G, Nicole G, Cody A, Anders O |
| Citation(s) |
40406518 |
| |
| Submission date |
Jan 21, 2025 |
| Last update date |
May 30, 2025 |
| Contact name |
Scott Stephen Grieshaber |
| E-mail(s) |
scottg@uidaho.edu
|
| Phone |
3525750394
|
| Organization name |
University of Idaho
|
| Department |
Biological Sciences
|
| Street address |
875 Perimeter Drive MS 3051
|
| City |
Moscow |
| State/province |
ID |
| ZIP/Postal code |
83844-3051 |
| Country |
USA |
| |
|
| Relations |
| Reanalysis of |
GSM8173080 |
| Reanalysis of |
GSM8173079 |
| Reanalysis of |
GSM8173078 |
| Reanalysis of |
GSM8173083 |
| Reanalysis of |
GSM8173082 |
| Reanalysis of |
GSM8173081 |
| Reanalysis of |
GSM8173086 |
| Reanalysis of |
GSM8173085 |
| Reanalysis of |
GSM8173084 |
| Reanalysis of |
GSM8173089 |
| Reanalysis of |
GSM8173088 |
| Reanalysis of |
GSM8173087 |
| Reanalysis of |
GSM8173092 |
| Reanalysis of |
GSM8173091 |
| Reanalysis of |
GSM8173090 |
| Reanalysis of |
GSM8173095 |
| Reanalysis of |
GSM8173094 |
| Reanalysis of |
GSM8173093 |
| Reanalysis of |
GSM8173098 |
| Reanalysis of |
GSM8173097 |
| Reanalysis of |
GSM8173096 |
| Reanalysis of |
GSM8173101 |
| Reanalysis of |
GSM8173100 |
| Reanalysis of |
GSM8173099 |
| Reanalysis of |
GSM8173104 |
| Reanalysis of |
GSM8173103 |
| Reanalysis of |
GSM8173102 |
| Reanalysis of |
GSM8173107 |
| Reanalysis of |
GSM8173106 |
| Reanalysis of |
GSM8173105 |
| Reanalysis of |
GSM8499642 |
| Reanalysis of |
GSM8499643 |
| Reanalysis of |
GSM8499644 |
| Reanalysis of |
GSM8499645 |
| Reanalysis of |
GSM8499646 |
| Reanalysis of |
GSM8499647 |
| Reanalysis of |
GSM8499648 |
| Reanalysis of |
GSM8499649 |
