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| Status |
Public on Jan 31, 2025 |
| Title |
Novel insights into the genetic basis and proteotranscriptomic elucidation of virulence attenuation in Pseudomonas syringae pv. actinidiae variants |
| Organism |
Pseudomonas syringae pv. actinidiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of kiwifruit bacterial canker, but the factors affecting its pathogenicity in natural settings remain poorly explored. In this study, we isolated two Psa strains, G126 and G282 from infected kiwifruit orchards in Guizhou Province of China. Both isolates, categorized as Psa-biovar 3, were confirmed through Psa3-specific primers and phylogenomic analysis. Pathogenicity assays on kiwifruit cultivar ‘Hongyang’ leaves and branches showed significantly reduced numbers of necrotic spots and reduced lesions sizes upon infection with G282 compared to the G1 positive control strain, while G126 showed nonpathogenic phenotype. Additionally, both strains failed to induce a hypersensitive response in nonhost Nicotiana tabacum plants, and exhibited significantly reduced promoter activity of the hrpR/S, hrpL, and hrpA genes, which are crucial for the type III secretion system (T3SS). Genomic sequencing revealed that the T3SS of G126 was defective due to a single-nucleotide polymorphism in the hrpR gene, while G282 was entirely deficient in the type VI secretion system (T6SS), which potentially regulates the expression of T3SS genes. Transcriptomic analysis showed widespread alterations in key aspects of the secretion system, protein transport, and signal transduction, further supporting the phenotype characteristics exhibited by the strains. This study enhances our understanding of the genetic basis of nonpathogenic and partially pathogenic Psa isolates, highlighting the functional interdependencies between T3SS and T6SS.
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| Overall design |
RNA-seq profiling of wild-type strain G1， non-pathogenic strain G126 and Pathogenetic deficiency mutant G282 after 6-hour induction in HDM (hrp-derepressing medium) culture medium
Grant awarded to Zhao Zhibo: National Key Research and Development Program of China (Grant No. 2022YFD1400200)
Grant awarded to Zhao Zhibo: National Natural Science Foundation of China (Grant No. 31860486, 32001864)
Grant awarded to Zhao Zhibo: Fund for the China Agriculture Research System (Grant No. CARS-26-04A)
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| Contributor(s) |
Asif M, Hu R, Xie T, Zhao Z |
| Citation missing |
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| |
| Submission date |
Jan 07, 2025 |
| Last update date |
Jun 23, 2025 |
| Contact name |
Muhammad Asif |
| E-mail(s) |
m.asifssa@hotmail.com
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| Phone |
17800150073
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| Organization name |
Guizhou University
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| Department |
Plant Pathology
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| Street address |
college of agriculture, Guizhou university
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| City |
Guiyang |
| State/province |
Guizhou |
| ZIP/Postal code |
550025 |
| Country |
China |
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| Platforms (1) |
| GPL35271 |
Illumina NovaSeq 6000 (Pseudomonas syringae pv. actinidiae) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1207504 |
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