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Series GSE28515 Query DataSets for GSE28515
Status Public on Dec 01, 2011
Title Effect of acute ethanol on medidal prefrontal cortex across BXD genetic mapping panel and progenitors.
Organism Mus musculus
Experiment type Expression profiling by array
Summary In order to elucidate the molecular mechanisms underlying individual variation in sensitivity to ethanol we profiled the prefrontal cortex transcriptomes of two inbred strains that exhibit divergent responses to acute ethanol, the C57BL6/J (B6) and DBA/2J (D2) strains, as well as 27 members of the BXD recombinant inbred panel, which was derived from a B6 x D2 cross. With this dataset we were able to identify several gene co-expression networks that were robustly altered by acute ethanol across the BXD panel. These ethanol-responsive gene-enriched networks were heavily populated by genes regulating synaptic transmission and neuroplasticity, and showed strong genetic linkage to discreet chromosomal loci. Network-based measurements of node importance identified several hub genes as established regulators of ethanol response phenotypes, while other hubs represent novel candidate modulators of ethanol responses.
Overall design Animals were injected intraperitoneally (IP) with saline or 1.8 g/kg of ethanol. As part of a parallel study of ethanol induced anxiolysis, all mice underwent behavioral testing that included 15 minutes of restraint in a 50 mL conical tube followed by 10 minutes in a light-dark chamber. Mice were killed by cervical dislocation four hours following IP injection. Immediately thereafter, brains were extracted and chilled for 1 minute in iced phosphate buffer before being microdissected into 8 constituent regions, including the medial prefrontal cortex. Samples were randomly assigned to batch groups prior to total RNA extraction, cRNA synthesis and hybridization. Each microarray represent a pooling of 4-5 animals.
Contributor(s) Miles MF, Putman AH, Vorster PJ, Wolen AR
Citation(s) 22511924, 26948279
Submission date Apr 11, 2011
Last update date Feb 11, 2019
Contact name Michael Miles
Organization name Virginia Commonwealth Univ.
Department Pharmacology and Toxicology
Lab Miles
Street address PO Box 980613
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (66)
GSM706288 pfc_sal_c57_rep1
GSM706289 pfc_sal_c57_rep2
GSM706290 pfc_sal_c57_rep3
BioProject PRJNA138977

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28515_RAW.tar 397.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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