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Series GSE28412 Query DataSets for GSE28412
Status Public on Apr 07, 2011
Title Analyzing the metabolic stress response of recombinant Escherichia coli cultures expressing human interferon beta in high cell density fed batch cultures using time course transcriptomic data
Organism Escherichia coli
Experiment type Expression profiling by array
Summary Over expression of recombinant proteins is known to cause a metabolic burden to the host cells which leads to down regulation of both growth rates and protein expression. Most studies in this regard have been conducted in low density shake flask cultures which does not capture the essential features of an industrial scale bioprocess. In the present work we studied the transcriptomic profiling at different specific growth rates while expressing the recombinant human interferon beta in fed batch cultures with complex media. These conditions mimicked the industrial fermentations for recombinant proteins.
Overall design To determine the transcriptomic response of E.coli BL21(DE3) to recombinant protein production at three different specific growth rates, samples were collected for four time points (0h, 2h, 4h, 6h) post induction for the fed batch cultivations at specific growth rates 0.2h-1, 0.3h-1, 0.4h-1 respectively and induced at an optical density of 30. 0h (uninduced) sample was taken as control conditions for every run.
Contributor(s) Singh AB, Sharma AK, Mukherjee KJ
Citation(s) 22018139
Submission date Apr 06, 2011
Last update date Mar 08, 2019
Contact name Krishna Jyoti Mukherjee
Phone 011-26704087
Organization name Jawaharlal Nehru University
Department School Of Biotechnology
Lab Bioprocess and Biosystems Enginerring Laboratory
Street address New Mehrauli Road
City New Delhi
State/province delhi
ZIP/Postal code 110067
Country India
Platforms (1)
GPL3154 [E_coli_2] Affymetrix E. coli Genome 2.0 Array
Samples (12)
GSM702192 specific growth rate 0.2h-1 0h
GSM702193 specific growth rate 0.2h-1 2h
GSM702194 specific growth rate 0.2h-1 4h
BioProject PRJNA139171

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28412_RAW.tar 12.5 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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