 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 05, 2011 |
| Title |
Genome-wide identification of DAF-12 binding sites in Caenorhabditis elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
DAF-12 is involved in development, dauer formation, and aging in Caenorhabditis elegans. To understand how DAF-12 is involved in these biologic processes, we performed chIP-chip using a DAF-12:TAP transgene and an anti-DAF-12 antibody. We identified 1175 genomic binding sites which were located within 5 kb of 3179 genes. These genes include known DAF-12 target genes as well as new genes involved in developmental timing, microRNA function, and stress resistance.
The supplemental BED file contains all 1175 genomic binding sites described in our work.
|
| |
|
| Overall design |
DAF-12:TAP transgenic worms with chIP performed using an anti-DAF-12 antibody compared to input chromatin and chIP performed using the daf-12(rh61rh411) null mutant.
|
| |
|
| Contributor(s) |
Hochbaum D, Zhang Y, Stuckenholz C, Labhart P, Alexiadis V, Martin R, Knölker H, Fisher AL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 04, 2011 |
| Last update date |
Apr 30, 2013 |
| Contact name |
Alfred L Fisher |
| E-mail(s) |
fishera@dom.pitt.edu
|
| Phone |
412-383-5892
|
| Fax |
412-692-2370
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine
|
| Lab |
Fisher lab
|
| Street address |
3471 5th Ave., Suite 500
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15260 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5634 |
[Ce25b_MR] Affymetrix C. elegans Tiling 1.0R Array |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA139461 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28350_INPUT.CEL.gz |
28.3 Mb |
(ftp)(http) |
CEL |
| GSE28350_RAW.tar |
181.1 Mb |
(http)(custom) |
TAR (of BAR, BED, CEL) |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...