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| Status |
Public on Dec 09, 2024 |
| Title |
Incongruent end structures of leading and lagging telomeres dictate the nature of end replication problem |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
The end replication problem refers to the incomplete replication of parental DNA at telomeres, a process whose molecular depiction is hampered by the complex nature of telomere ends. Here we recapitulate this process using a synthetic de novo telomere in yeast and delineate distinct molecular fates of telomere ends in vivo. We show that the lagging strand telomere carries a ~10 nt 3’ overhang, while the leading strand telomere has a Yku-protected blunt end, which is prevalent on native telomeres. Moreover, we find that RNase H2 is mainly responsible for the removal of the last RNA primer. In the absence of RNase H2 activity, RNA primer retention on the lagging strand telomere attenuates telomere erosion and delays senescence in telomerase-null cells. These findings highlight incongruent end structures on telomeres and clarify that the primary culprit behind end replication problem is the incompletely replicated lagging strand telomere.
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| Overall design |
END-seq analysis of blunt ends on native telomeres
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| Contributor(s) |
Ye T, Yuan Q, Wu S, Zhao J, Wu Z, Liu J, Wu W, Zhou J |
| Citation missing |
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| |
| Submission date |
Dec 04, 2024 |
| Last update date |
Feb 26, 2025 |
| Contact name |
Qingqing Yuan |
| E-mail(s) |
yuanqingqing2023@sibcb.ac.cn
|
| Organization name |
CEMCS, CAS
|
| Department |
SIBCB
|
| Lab |
Wulab
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL33987 |
DNBSEQ-T7 (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1193986 |
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