NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE281995 Query DataSets for GSE281995
Status Public on Nov 28, 2024
Title Expression Analysis between HUVECs and EndMTed HUVECs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Prostate cancer often progresses to castration-resistant prostate cancer (CRPC), with neuroendocrine prostate cancer (NEPC) representing a highly aggressive variant. This study shows that endothelial-to-mesenchymal transition (EndMT) in vascular endothelial cells, induced by IL-1β and TGF-β2, enhances neuroendocrine differentiation in prostate cancer cells. LNCaP cells co-cultured with EndMTed HUVECs exhibited increased expression of neuroendocrine markers such as chromogranin A. GM-CSF emerged as a key mediator in this process, and its addition under androgen deprivation conditions further elevated neuroendocrine marker expression. Anti-androgen therapy with enzalutamide also paradoxically increased IL-1β and TGF-β2 secretion, promoting EndMT and subsequent neuroendocrine differentiation. These results highlight the potential of targeting EndMT and GM-CSF pathways as therapeutic strategies for aggressive, treatment-resistant forms of NEPC.
 
Overall design To investigate the factors excreted by EndMTed HUVECs, which might induce neuroendocrine differentiation of LNCaP cells, we performed a comprehensive expression analysis to compare differences in expression between HUVECs and EndMTed HUVECs by RNA-Seq.
 
Contributor(s) Kageyama T, Sekito S, Murakawa Y, Inoue T
Citation missing Has this study been published? Please login to update or notify GEO.
BioProject PRJNA1181033
Submission date Nov 15, 2024
Last update date Nov 28, 2024
Contact name Takumi Kageyama
E-mail(s) kagetaku@med.mie-u.ac.jp
Phone +81-59-231-5026
Organization name Mie University Graduate School of Medicine
Department Department of Nephro-Urologic Surgery and Andrology
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM8634120 normal HUVEC 1
GSM8634121 normal HUVEC 2
GSM8634122 normal HUVEC 3

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE281995_counts.txt.gz 5.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap