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| Status |
Public on Nov 28, 2024 |
| Title |
Expression Analysis between HUVECs and EndMTed HUVECs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Prostate cancer often progresses to castration-resistant prostate cancer (CRPC), with neuroendocrine prostate cancer (NEPC) representing a highly aggressive variant. This study shows that endothelial-to-mesenchymal transition (EndMT) in vascular endothelial cells, induced by IL-1β and TGF-β2, enhances neuroendocrine differentiation in prostate cancer cells. LNCaP cells co-cultured with EndMTed HUVECs exhibited increased expression of neuroendocrine markers such as chromogranin A. GM-CSF emerged as a key mediator in this process, and its addition under androgen deprivation conditions further elevated neuroendocrine marker expression. Anti-androgen therapy with enzalutamide also paradoxically increased IL-1β and TGF-β2 secretion, promoting EndMT and subsequent neuroendocrine differentiation. These results highlight the potential of targeting EndMT and GM-CSF pathways as therapeutic strategies for aggressive, treatment-resistant forms of NEPC.
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| Overall design |
To investigate the factors excreted by EndMTed HUVECs, which might induce neuroendocrine differentiation of LNCaP cells, we performed a comprehensive expression analysis to compare differences in expression between HUVECs and EndMTed HUVECs by RNA-Seq.
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| Contributor(s) |
Kageyama T, Sekito S, Murakawa Y, Inoue T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA1181033 |
| |
| Submission date |
Nov 15, 2024 |
| Last update date |
Nov 28, 2024 |
| Contact name |
Takumi Kageyama |
| E-mail(s) |
kagetaku@med.mie-u.ac.jp
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| Phone |
+81-59-231-5026
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| Organization name |
Mie University Graduate School of Medicine
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| Department |
Department of Nephro-Urologic Surgery and Andrology
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| Street address |
2-174 Edobashi
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| City |
Tsu |
| State/province |
Mie |
| ZIP/Postal code |
514-8507 |
| Country |
Japan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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