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Series GSE28166 Query DataSets for GSE28166
Status Public on Sep 02, 2011
Title Host Regulatory Network Response to Infection with Highly Pathogenic H5N1 Avian Influenza Virus
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Over the last decade, more than half of humans infected with highly pathogenic avian influenza (HPAI) H5N1 viruses have died, and yet virus-induced host signaling has yet to be clearly elucidated. Airway epithelia are known to produce inflammatory mediators that contribute to HPAI H5N1-mediated pathogenicity, but a comprehensive analysis of the host response in this cell type is lacking. Here, we leveraged a systems biology method called weighted gene correlation network analysis (WGCNA) to identify and statistically validate signaling sub-networks that define the dynamic transcriptional response of human bronchial epithelial cells after infection with influenza A/Vietnam/1203/2004 (H5N1, VN1203). A detailed examination of two sub-networks involved in the immune response and keratin filament formation revealed potential novel mediators of HPAI H5N1 pathogenesis, and additional experiments validated upregulation of these transcripts in response to VN1203 infection in C57BL/6 mice. Using emergent network properties, we provide fresh insight into the host response to HPAI H5N1 virus infection, and identify novel avenues for perturbation studies and potential therapeutic intervention of fatal HPAI H5N1 disease.
Overall design Calu-3 cells were infected with VN1203 influenza virus and profiled at 0, 3, 7, 12, 18, and 24 hours post infection. There are 3 mock and infected replicates for each time point.
Contributor(s) Li C, Bankhead III A, Eisfeld AJ, Hatta Y, Jeng S, Chang JH, Aicher LD, Law GL, Waters K, Neumann G, Katze MG, McWeeney S, Kawaoka Y
Citation(s) 21865398
Submission date Mar 24, 2011
Last update date Jan 23, 2019
Contact name Armand Bankhead III
Organization name Oregon Health and Science University
Department Depatartment of Medical Informatics and Clinical Epidemiology
Street address 3181 SW Sam Jackson Park Rd.
City Portland
State/province OR
ZIP/Postal code 97080
Country USA
Platforms (1)
GPL6480 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version)
Samples (36)
GSM697564 Mock_0H_1
GSM697565 Mock_0H_2
GSM697566 Mock_0H_3
BioProject PRJNA139513

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28166_RAW.tar 320.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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