|
| Status |
Public on Apr 12, 2012 |
| Title |
Psip1 ChIP using A300-847 antibody (Immunoprecipitates mainly p52 isoform of Psip1) Chip-chip from Mouse MEFs cells with H3K36me3, H3K4me3, Psip1 ChIP using A300-847 antibody |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing.
Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and H3K4me3 and by ChIP-on-chip analysis demonstrated that, Like H3K36me3, Psip1 is enriched on exons of highly expressed genes,
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| |
|
| Overall design |
Comparision of H3K36me3 and Psip1 binding sites on the expressed and non expressed genes.
|
| |
|
| Contributor(s) |
Marulasiddappa PM, Grimes GR, Bickmore WA |
| Citation(s) |
22615581 |
| Submission date |
Mar 24, 2011 |
| Last update date |
May 29, 2012 |
| Contact name |
Pradeepa M Madapura |
| E-mail(s) |
p.m.madapura@qmul.ac.uk, manthankumar.patel@qmul.ac.uk
|
| Organization name |
Queen Mary University of London
|
| Lab |
Pradeepa Madapura
|
| Street address |
4 Newark Street, Whitechapel
|
| City |
London |
| State/province |
UK |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL13276 |
NimbleGen Custom Mouse Hox Tiling Array |
|
| Samples (10)
|
| GSM697402 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep1 |
| GSM697403 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep2 |
| GSM697404 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep3 |
| GSM697405 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep4 |
| GSM697406 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep1 |
| GSM697407 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep2 |
| GSM697408 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep3 |
| GSM697409 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep4 |
| GSM697410 |
anti H3K4me3 (Millipore 07-473) |
| GSM697411 |
anti H3K4me3 (Millipore 07-473) in Psip1 mutant |
|
| Relations |
| BioProject |
PRJNA139395 |
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