|
Status |
Public on Apr 12, 2012 |
Title |
Psip1 ChIP using A300-847 antibody (Immunoprecipitates mainly p52 isoform of Psip1) Chip-chip from Mouse MEFs cells with H3K36me3, H3K4me3, Psip1 ChIP using A300-847 antibody |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
Summary |
The p52 isoform of Psip1/Ledgf links histone H3K36 methylation and the regulation of alternative splicing. Chromatin immunoprecipitation (ChIP) of Psip1 together with H3K36me3 and H3K4me3 and by ChIP-on-chip analysis demonstrated that, Like H3K36me3, Psip1 is enriched on exons of highly expressed genes,
|
|
|
Overall design |
Comparision of H3K36me3 and Psip1 binding sites on the expressed and non expressed genes.
|
|
|
Contributor(s) |
Marulasiddappa PM, Grimes GR, Bickmore WA |
Citation(s) |
22615581 |
Submission date |
Mar 24, 2011 |
Last update date |
May 29, 2012 |
Contact name |
Pradeepa M Madapura |
E-mail(s) |
p.m.madapura@qmul.ac.uk, manthankumar.patel@qmul.ac.uk
|
Organization name |
Queen Mary University of London
|
Lab |
Pradeepa Madapura
|
Street address |
4 Newark Street, Whitechapel
|
City |
London |
State/province |
UK |
ZIP/Postal code |
E1 2AT |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL13276 |
NimbleGen Custom Mouse Hox Tiling Array |
|
Samples (10)
|
GSM697402 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep1 |
GSM697403 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep2 |
GSM697404 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep3 |
GSM697405 |
Psip1 ChIP using A300-847 antibody (recognises p52 isoform of Psip1) rep4 |
GSM697406 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep1 |
GSM697407 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep2 |
GSM697408 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep3 |
GSM697409 |
H3K36me3 ChIP using anti H3K36me3 (Abcam Ab 9050-100) rep4 |
GSM697410 |
anti H3K4me3 (Millipore 07-473) |
GSM697411 |
anti H3K4me3 (Millipore 07-473) in Psip1 mutant |
|
Relations |
BioProject |
PRJNA139395 |