 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 11, 2024 |
| Title |
Human subcutaneous and visceral adipocyte atlases uncover classical and non-classical adipocytes and depot-specific patterns |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human adipose depots are functionally distinct. Yet, recent single-nucleus RNA-sequencing (snRNA-seq) analyses largely uncovered overlapping/similar cell-type landscapes. We hypothesized that adipocytes subtypes, differentiation trajectories, and/or intercellular communication patterns could illuminate this depot similarity-difference gap. For this, we performed snRNA-seq of human subcutaneous/visceral adipose tissues (5/10 samples, respectively). Of 27,665 adipocyte nuclei in both depots, the majority were “classical”, namely- enriched in lipid metabolism pathways. However, we also observed “non-classical” adipocyte subtypes, enriched in immune-related, extracellular matrix deposition (fibrosis), vascularization/angiogenesis, or ribosomal processes. Pseudo-temporal analysis showed a developmental trajectory from adipose progenitor cells to classical adipocytes via non-classical adipocytes, suggesting that the classical state stems from loss, rather than gain, of specialized functions. Lastly, intercellular communication routes were consistent with the different inflammatory tone of the two depots. Jointly, these findings provide a high-resolution view into the contribution of cellular composition, differentiation, and intercellular communication patterns to human fat depot differences.
|
| |
|
| Overall design |
snRNA-seq of visceral fat tissue, subcutaneous adipose tissue tissue from 10 human donors.
UPDATE: [Jan-11-2025] In Sample GSM8619168, the sex, ethnicity, and age of donor 3313 were updated.
|
| |
|
| Contributor(s) |
Yeger-Lotem E, Rudich A, Lazarescu O, Ziv-Agam M, Haim Y, Hekselman I, Jubran J, Shneyour A, Muallem H, Zemer A, Rosengarten-Levin M, Kitsberg D, Levin L, Liberty IF, Yoel U, Dukhno O, Adam M, Braune J, Müller C, Raulien N, Gericke M, Körner A, Murphy R, Blüher M, Habib N |
| Citation(s) |
39856219 |
| |
| Submission date |
Nov 07, 2024 |
| Last update date |
Feb 25, 2025 |
| Contact name |
Lattice Data Coordination |
| E-mail(s) |
lattice-info@lists.stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Street address |
3165 Porter Drive
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA1183538 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE281356_RAW.tar |
445.5 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...