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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 30, 2024 |
| Title |
Spatially-restricted and ontogenically-distinct hepatic macrophages are required for tissue repair. |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Analysis of single cell RNA-seq, CITE-seq data and bulk RNA-seq data on cells isolated from the livers of mice following PBS and APAP (paracetamol/acetaminophen) administration. Spatial Transcriptomics analysis was performed using 10x Visium platform on mouse livers 48hrs post PBS or APAP administration. We also analysed single cell RNA-seq data on CD64+F4/80+ macrophages isolated from the livers of Trem2 fl/fl mice and CD64CRE Trem2 fl/fl mice.
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| Overall design |
Single cell RNA Seq = Total liver cells derived from mice following PBS and APAP administration and CD64+F4/80+ macrophages from Trem2 fl/fl mice and CD64CRE Trem2 fl/fl mice.
Bulk RNA seq on KCs and LAM-like KCs isolated from livers 48hr post PBS or APAP administration
10 Visium Spatial Seq = mouse liver 48h post PBS or APAP administration.
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| Web link |
https://livercellatlas.org/
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| Contributor(s) |
De Ponti FF, Bujko A, Liu Z, Collins PJ, Schuermans S, Maueroder C, Amstelveen S, Thoné T, Martens L, McKendrick JG, Louwe PA, Sànchez Cruz A, Saelens W, Matchett KP, Waller KJ, Zwicker C, Buglar-Lamb A, Vanneste B, Parmentier F, Binte Abdul Latib M, Remmerie A, Kertesz L, Kremer A, Verbeke J, Højland Ipsen D, Reinhard Pfister D, Liu Z, Guilliams M, Henderson NC, Ravichandran K, Marques PE, Scott C |
| Citation(s) |
39862865 |
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| Submission date |
Oct 29, 2024 |
| Last update date |
Jan 29, 2025 |
| Contact name |
Liesbet Martens |
| E-mail(s) |
liesbet.martens@irc.vib-ugent.be
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| Organization name |
VIB-University of Ghent
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| Department |
VIB Inflammation Research Center
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| Street address |
Technologiepark 927
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| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1179316 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE280515_RAW.tar |
28.1 Mb |
(http)(custom) |
TAR (of CSV, H5, JSON, PNG) |
SRA Run Selector |
| Raw data are available in SRA |
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