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| Status |
Public on Jan 19, 2025 |
| Title |
TGFβ signaling controls neural crest developmental plasticity via Smad2/3 [ATAC-seq] |
| Organism |
Gallus gallus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The neural crest is a highly plastic stem cell population that represents a notable exception to the germ layer theory. Despite being of ectodermal origin, these cells can differentiate into skeletal derivatives like cartilage and bone, tissues that are typically formed by mesoderm. The inductive program that endows the neural crest with these unique properties is still poorly understood. Here, we report that Smad2/3-mediated TGFβ signaling endows cranial neural crest cells with enhanced developmental potential. Our results show that TGFβ signaling modulates neural crest axial identity and directly activates the gene circuits that support skeletal differentiation. Cooperation between TGFβ and low levels of WNT-signaling in the embryonic head activates cranial-specific cis-regulatory elements of anterior genes. Activation of the TGFβ pathway allowed reprogramming of trunk neural crest cells to adopt an anterior identity and led to the development of an improved protocol for the generation of human cranial neural crest cells. Our findings indicate TGFβ signaling is required for the specification of cranial neural crest cells, endowing them with the potential to give rise to the craniofacial skeleton.
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| Overall design |
We performed ATAC-seq analysis on neural crest explant cultures under different TGFB1 and CHIR99021 treatment conditions.
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| Contributor(s) |
Rothstein M, Azambuja A, Kanno T, Breen C, Simoes-Costa M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 25, 2024 |
| Last update date |
Jan 20, 2025 |
| Contact name |
Megan Rothstein |
| E-mail(s) |
mmr234@cornell.edu
|
| Organization name |
Cornell University
|
| Street address |
526 Campus Road Rm 433
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE175811 |
TGFβ signaling controls neural crest developmental plasticity via Smad2/3 |
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| Relations |
| BioProject |
PRJNA1165405 |
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