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| Status |
Public on Sep 12, 2024 |
| Title |
TCA metabolism regulates DNA hypermethylation in LPS and Mycobacterium tuberculosis–induced immune tolerance |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Severe and chronic infections, including pneumonia, sepsis, and tuberculosis (TB), induce long-lasting epigenetic changes that are associated with an increase in all cause post-infectious morbidity and mortality. We show that TCA cycle also regulates epigenetics, specifically DNA methylation, after infection induced immune tolerance. The following data is DNA methylation from lipopolysaccharide (LPS) in presence of by inhibitors of cellular metabolism (rapamycin, everolimus, metformin) and the TCA cycle (IDH inhibitors). DNA methylation profiles were also generated from exogenous supplementation with TCA metabolites (succinate and itaconate) in Monocyte derived Macrophages
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| Overall design |
Bisulphite converted DNA from the four sample across 6 conditions; 23samples and 6 samples from metabolite treated cells total 29 samples were hybridised to the Illumina EPIC 850K
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| Contributor(s) |
Abhimanyu ., Longlax SC, Nishiguchi T, Ladki M, Sheikh D, Martinez AL, Mace EM, Grimm S, Caldwell T, Portillo Varela A, Sekhar RV, Mandalakas AM, Mlotshwa M, Ginidza S, Cirillo JD, Wallis RS, Netea MG, van Crevel R, Coarfa C, DiNardo AR |
| Citation(s) |
39348545 |
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| Submission date |
Sep 11, 2024 |
| Last update date |
Oct 02, 2024 |
| Contact name |
Abhimanyu - |
| E-mail(s) |
Abhimanyua@uchicago.edu
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| Organization name |
Baylor College of Medicine
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| Lab |
DiNardo Lab
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| Street address |
one Baylor Plaza
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA1159897 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE276988_Matrix_processed.txt.gz |
141.7 Mb |
(ftp)(http) |
TXT |
| GSE276988_Matrix_signal_intensities.txt.gz |
147.2 Mb |
(ftp)(http) |
TXT |
| GSE276988_RAW.tar |
570.7 Mb |
(http)(custom) |
TAR (of IDAT) |
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