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| Status |
Public on Sep 15, 2024 |
| Title |
scRNA-seq of spleen cells from mice immunized with AALI or LNP for monkeypox vaccine development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We developed two multivalent mRNA vaccines that induced strong immune responses and provided protection against monkeypox virus in mice. Additionally, we used single-cell RNA sequencing and V(D)J sequencing to explore the postvaccination immune landscape at the single-cell level and revealed B-cell receptor and T-cell receptor diversity, gene rearrangement, and predicted CDR3 motifs, systematically exploring the post-vaccination immune landscape at the single-cell level. These findings are poised to guide future vaccine design and present an innovative clinical strategy against monkeypox and orthopoxvirus outbreaks.
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| Overall design |
We developed two multivalent mRNA vaccine candidates, AAL and AALI. Using a mouse infection model established with Clade IIb MPXV isolated from clinical patients, we employed single-cell RNA sequencing (scRNA-seq) and V(D)J sequencing to investigate the transcriptional and immune landscapes of B and T cells following vaccination.
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| Contributor(s) |
Li Y, Cheng L, Jiang L, Chen S |
| Citation missing |
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| BioProject |
PRJNA1156919 |
| |
| Submission date |
Sep 08, 2024 |
| Last update date |
Sep 16, 2024 |
| Contact name |
Yansheng Li |
| E-mail(s) |
lixusophia@gmail.com
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| Organization name |
Shenzhen Second People's Hospital
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| Street address |
3002 Sungang West Road, Futian District
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| City |
Shenzhen |
| ZIP/Postal code |
518060 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM8503862 |
scRNA-seq of mice_1 immunized with AALI |
| GSM8503863 |
scRNA-seq of mice_2 immunized with AALI |
| GSM8503864 |
scRNA-seq of mice_3 immunized with AALI |
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