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Series GSE27546 Query DataSets for GSE27546
Status Public on Feb 28, 2011
Title Effects of HMGN variants on cellular transcription profile
Organism Mus musculus
Experiment type Expression profiling by array
Summary HMGN (high mobility group N) is a family of intrinsically disordered nuclear proteins that binds to nucleosomes, alters the structure of chromatin and affects transcription. A major unresolved question is the extent of functional specificity, or redundancy, between the various members of the HMGN protein family.
Here we analyze the transcriptional profile of cells in which the expression of various HMGN proteins has been either deleted or doubled. The results reveal an HMGN-variant specific effect on the fidelity of the cellular transcription profile, indicating that functionally, the various HMGN subtypes are not fully redundant.
 
Overall design RNA was collected from either primary knock-out MEFs or SV40-transformed MEFs and MIN6 cells over expressing various HMGN proteins and mutants and hybridized to Affymetrix arrays. We obtained a double ammount of HMGN proteins in MEFs and MIN6 cells by retroviral infection and subsequent selection procedure. We collected all infected cells (pools, not clones) in order to eliminate the effect of viral integration in the genome.
 
Contributor(s) Taher L, Rochman M
Citation(s) 21278158
Submission date Feb 27, 2011
Last update date Feb 11, 2019
Contact name Leila Taher
E-mail leila.taher@uni-rostock.de
Organization name University of Rostock
Street address Ernst-Heydemann-Str. 8
City Rostock
ZIP/Postal code 18057
Country Germany
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (51)
GSM680215 MEFs HMGN1 knock out rep 1
GSM680216 MEFs HMGN1 knock out rep 2
GSM680217 MEFs HMGN1 knock out rep 3
Relations
BioProject PRJNA137095

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27546_RAW.tar 158.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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