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| Status |
Public on Sep 06, 2024 |
| Title |
Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We examined the transcriotional changes in antigen specific CD4 T cells to antigens derived from thymus or to the antigens derived from islets in the autoimmune diabetes mouse model.
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| Overall design |
Single cells were prepared from spleen and lymph nodes of 8-12 weeks old NOD mice or HEL specfic TCR transgenic mice. Tetramer enriched cells were sorted from the NOD mice and naïve CD4 T cells were sorted from HEL TCR trangenic mice. Before surface staining, samples were incubated with 100 μl of TotalSeq™ anti-mouse Hashtag reagent (1:100 [vol/vol], BioLegend) to tag the samples: enriched cells from the islet epitope set were tagged with the TotalSeq-C0301 anti-mouse Hashtag 1 Antibody (Barcode sequence: ACCCACCAGTAAGAC); enriched cells from the thymic epitope set were tagged with the TotalSeq-C0302 anti-mouse Hashtag 2 Antibody (Barcode sequence: GGTCGAGAGCATTCA); 10E11 naïve CD4+ T cells were tagged with the TotalSeq-C0303 anti-mouse Hashtag 3 Antibody (Barcode sequence: CTTGCCGCATGTCAT).
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| Contributor(s) |
Hu H, Li T, Zhang B, Wan X |
| Citation(s) |
39333495 |
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| Submission date |
Aug 19, 2024 |
| Last update date |
Dec 12, 2024 |
| Contact name |
Tiandao Li |
| Organization name |
Washington University
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| Street address |
4444 Forest Park Ave
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| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM8472799 |
Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 1 |
| GSM8472800 |
Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 2 |
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| Relations |
| BioProject |
PRJNA1149809 |
