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| Status |
Public on Sep 13, 2024 |
| Title |
The Regulation of MicroRNA-21 by Interleukin-6 and its Role in the Development of Fibrosis in Endometriotic Lesions |
| Organism |
Papio anubis |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Endometriosis is one of the most common causes of chronic pelvic pain and infertility that affects 10% of women of reproductive age. It is currently defined as the presence of endometrial epithelial and stromal cells at ectopic sites; however, advances in endometriosis research have some authors believing that endometriosis should be re-defined as “a fibrotic condition in which endometrial stroma and epithelium can be identified”. microRNAs (miRNAs) are regulatory molecules that potentially play a role in endometriotic lesion development. There is evidence that suggests that miRNAs, including microRNA-21 (miR-21), participate in fibrotic processes in different organs, including the heart, kidney, liver and lungs. The objective of this study was to understand the role of miR-21 and the mechanisms that can contribute to the development of fibrosis by determining how IL-6 regulates miR-21 expression and how this miRNA regulates the transforming growth factor beta (TGF-β) signaling pathway to promote fibrosis. We investigated the expression of miR-21 in the baboon and mouse model of endometriosis and its correlation with fibrosis. We demonstrated that inflammation and fibrosis are present at a very early stage of endometriosis and that the inflammatory environment in the peritoneal cavity, which includes interleukin 6 (IL-6), can regulate the expression of miR-21 in vitro and in vivo.
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| Overall design |
Total RNA was extracted from the endometrium and endometriotic lesions from baboons with induced endometriosis for 15 months (n = 16) or spontaneous disease (n = 8). Small-RNA library preparation and high-throughput sequencing generated an average of 8.9 million reads per sample. Quality and adapter-trimmed reads (Trim Galore v0.3.3) were mapped to human miRNAs from miRbase (release 22) using the miRDeep2 (v0.0.7) pipeline. Differential expression analysis was conducted with edgeR (v3.22.5) using the edgeR-robust method.
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| Contributor(s) |
Bernal MA, Song Y, Joshi N, Burns GW, Paul EN, Vegter E, Hrbek S, Sempere LF, Fazleabas AT |
| Citation(s) |
39201680 |
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| Submission date |
Aug 15, 2024 |
| Last update date |
Sep 13, 2024 |
| Contact name |
Gregory Burns |
| E-mail(s) |
burnsgr2@msu.edu
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| Organization name |
Michigan State University
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| Department |
Obstetrics, Gynecology and Reproductive Biology
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| Lab |
Fazleabas
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| Street address |
400 Monroe Ave NW
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| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
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| Platforms (1) |
| GPL23487 |
Illumina HiSeq 2500 (Papio anubis) |
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| Samples (24)
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| GSM8461711 |
Lesion, 15 month, E07 |
| GSM8461712 |
Eutopic Endometrium, 15 month, E12 |
| GSM8461713 |
Lesion, 15 month, E11 |
| GSM8461714 |
Eutopic Endometrium, 15 month, E16 |
| GSM8461715 |
Lesion, 15 month, E15 |
| GSM8461716 |
Eutopic Endometrium, 15 month, E19 |
| GSM8461717 |
Lesion, 15 month, E18 |
| GSM8461718 |
Eutopic Endometrium, 15 month, E22 |
| GSM8461719 |
Lesion, 15 month, E21 |
| GSM8461720 |
Eutopic Endometrium, 15 month, E25 |
| GSM8461721 |
Lesion, 15 month, E24 |
| GSM8461722 |
Eutopic Endometrium, 15 month, E28 |
| GSM8461723 |
Lesion, 15 month, E27 |
| GSM8461724 |
Eutopic Endometrium, Spontaneous, S02 |
| GSM8461725 |
Lesion, Spontaneous, S01 |
| GSM8461726 |
Eutopic Endometrium, Spontaneous, S03 |
| GSM8461727 |
Lesion, Spontaneous, S04 |
| GSM8461728 |
Eutopic Endometrium, Spontaneous, S06 |
| GSM8461729 |
Lesion, Spontaneous, S05 |
| GSM8461730 |
Eutopic Endometrium, Spontaneous, S08 |
| GSM8461731 |
Lesion, Spontaneous, S07 |
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| Relations |
| BioProject |
PRJNA1148484 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE274922_miRNAs_expressed_all_samples_1537378662.xlsx |
299.3 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
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