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| Status |
Public on Jan 21, 2025 |
| Title |
snRNA-seq of murine tarsal plates |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The eyelid is a complex environment containing, containing epidermal, hair follicles, and conjunctival cells closely adjacent to the Meibomian glands (MGs), making it challenging to isolate pure MG cells or to identify MG contributions in bulk RNA-seq analyses. To analyze transcription in MG subpopulations, and to overcome difficulties in isolating cytoplasmic mRNAs from lipid-rich acinar cells, we utilized snRNA-seq which allows for identification of cell sub-populations within a complex mix.
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| Overall design |
The dorsal portion of the eyelids and the surrounding tissues near the tarsal plates were removed using a scalpel. The tarsal plates were digested with collagenase A and the MGs were dissected using forceps. Nuclei were analyzed from the pooled samples of four 8-week-old and four 21-month-old male mice using two replicates for each age (16 mice analyzed in total).
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| Contributor(s) |
Zhu X, Xu M, Millar SE |
| Citation(s) |
39955307 |
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| Submission date |
Aug 10, 2024 |
| Last update date |
Mar 11, 2025 |
| Contact name |
Sarah E Millar |
| E-mail(s) |
sarah.millar@mssm.edu
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| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Developmental, Cell and Regenerative Biology
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| Street address |
Icahn Building, Floor 13 Room 020C, 1425 Madison Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| GSM8450395 |
tarsal plates, 8 weeks wildtype, replicate 1 |
| GSM8450396 |
tarsal plates, 8 weeks wildtype, replicate 2 |
| GSM8450397 |
tarsal plates, 21 months wildtype, replicate 1 |
| GSM8450398 |
tarsal plates, 21 months wildtype, replicate 2 |
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| Relations |
| BioProject |
PRJNA1146742 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE274498_RAW.tar |
266.5 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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