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| Status |
Public on Jan 21, 2025 |
| Title |
Spatial transcriptomics of murine eyelid |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Meibomian glands (MGs) are specialized holocrine sebaceous glands embedded in the tarsal plate of the eyelids. The cell heterogeneity of MGs is not well characterized. In this study, we performed a spatial transcriptomic analysis of murine eyelid using the 10X Visium platform.
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| Overall design |
A pair of upper and lower eyelids from an 8-week-old male C57BL/6J mouse was dissected, sectioned at 10 μm, and mounted onto the assay slide containing four capture areas (6.5mm x 6.5mm) with spatially barcoded poly T capture probes. The subsequent procedures were conducted using the 10x Visium Spatial Gene Expression kit, according to the manufacturer’s instructions.
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| Contributor(s) |
Zhu X, Xu M, Millar SE |
| Citation(s) |
39955307 |
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| Submission date |
Aug 10, 2024 |
| Last update date |
Mar 11, 2025 |
| Contact name |
Sarah E Millar |
| E-mail(s) |
sarah.millar@mssm.edu
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| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Developmental, Cell and Regenerative Biology
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| Street address |
Icahn Building, Floor 13 Room 020C, 1425 Madison Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
| GSM8450394 |
mouse eyelid, 8 weeks wildtype [Spatial transcriptomics] |
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| Relations |
| BioProject |
PRJNA1146741 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE274497_RAW.tar |
10.4 Mb |
(http)(custom) |
TAR (of CSV, JPG, JSON, MTX, PNG, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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