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| Status |
Public on Sep 13, 2024 |
| Title |
Ulp1 Regulates Cell Proliferation through INO1 in Pichia pastoris |
| Organism |
Komagataella pastoris |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pichia pastoris is extensively employed as a host for expressing foreign proteins, and its secretion system is especially advantageous for efficient protein purification. Ulp1 is essential for the cell cycle. In Saccharomyces cerevisiae, the absence of Ulp1 leads to G2/M cell cycle arrest. Similarly, in Pichia pastoris, we also observed G2 phase stalling in Ulp1 knockout cells. RNA-seq data revealed significant changes in seven genes, including INO1. Interestingly, overexpressing INO1 in Ulp1 knockout cells restored cell proliferation, suggesting that Ulp1 regulates cell proliferation through INO1. However, adding extra inositol to Ulp1 knockout cells was not able to restore cell proliferation, indicating that the enzyme activity of INO1 is not essential for cell proliferation. Our data provide new insights into Ulp1 functions in the cell cycle and raise new questions about yeast cell cycle regulation.
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| Overall design |
Transcriptome sequencing and analysis results of wild-type Pichia pastoris X33 strain, Pichia pastoris x33 Ulp1 knockout strain, Pichia pastoris X33 Ulp1 knockout strain overexpressing INO1 strain, Pichia pastoris X33 Ulp1 knockout strain supplemented with inositol culture in culture medium.
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| Contributor(s) |
Yang J, Zhong B, Yang L, Chen G |
| Citation missing |
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| Submission date |
Aug 08, 2024 |
| Last update date |
Sep 14, 2024 |
| Contact name |
bo zhong |
| E-mail(s) |
zhongbo990509@gmail.com
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| Organization name |
Zhejiang Sci-Tech University
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| Street address |
No. 928, 2nd Street, Xiasha Higher Education Park, Qiantang District, Hangzhou City, Zhejiang Province
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| City |
hangzhou |
| State/province |
zhejiang |
| ZIP/Postal code |
310018 |
| Country |
China |
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| Platforms (1) |
| GPL29092 |
Illumina NovaSeq 6000 (Komagataella pastoris) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA1145913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE274307_RAW.tar |
12.3 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
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