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Series GSE273048 Query DataSets for GSE273048
Status Public on Jan 10, 2025
Title Spatial analysis of young and old murine lungs exposed to ozone and fibrogenic stress [spatial_ozone]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Ozone (O3) is a ubiquitous pollutant known to produce acute and transient inflammation through oxidative injury and inflammation. These effects are exacerbated in susceptible populations, such as the elderly and those exhibiting genetic mutations in central nodes of pulmonary function. To comprehend the impact of these predisposing factors, the present study examines structural, mechanical, and immunological responses to single acute O3 exposure (0.8 ppm, 3h) in young (12 week old) and old (80 week old) mice. We therefore examined the impact of ozone in a surfactant competent and mutant murine line. The latter models a clinically relevant mutation in the gene encoding for the alveolar epithelial type 2 specific surfactant protein C. Ozone exposure produced focal injury localized at the terminal bronchiole-to-alveolar junctions and enlarged alveoli in aged SP-C mutant lungs. This histological feature was used to select regions of interest for spatial transcriptomics. Paraffin sections of 4 mice were prepared: a young/12wk SP-C wild type air control, a young/12wk SP-C wild type ozone (0.8 ppm, 3h), a young/12wk SP-C mutant ozone, and an old/80wk ozone. Immunofluorescence for DNA, CD45, CD68 was performed to identify macrophages (CD45+CD68+) and non-immune ("other", DNA+CD45-) cells within healthy (air control only), peri-injured and injured regions of the lung. A total of 24 regions (680um x 700um in size) were selected from CD68+ macrophages and CD45- non immune/"other" cells. RNA sequencing was then performed. Principal component and pathway analysis revealed spatial heterogeneity in response to ozone, as well as a senescence signature in the aging lungs. These results illustrate regional and age dependent heterogeneity in immune and non-immune cells of the lung during an acute exposure to air pollution.
 
Overall design 4 sections were prepared: young/12wk SP-C wild type air control, a young/12wk SP-C wild type ozone (0.8 ppm, 3h), a young/12wk SP-C mutant ozone, and an old/80wk ozone - spatial analysis of CD45+CD68+ macrophages and DNA+CD45- non-immune ("other") cells.
Web link https://pubmed.ncbi.nlm.nih.gov/39832482/
 
Contributor(s) Venosa A, Noll J
Citation(s) 39832482
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 ES032553 Role of Surfactant Protein-C Mutation and Ozone Exposure in the Exacerbation of Pulmonary Fibrosis UNIVERSITY OF UTAH Alessandro Venosa
Submission date Jul 24, 2024
Last update date Apr 11, 2025
Contact name Alessandro Venosa
E-mail(s) Alessandro.venosa@pharm.utah.edu
Organization name University of Utah
Department Pharmacology and Toxicology
Street address 30 2000 E, Rm. 3952
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (50)
GSM8417637 no template control #1
GSM8417638 injured region 1_young ozone SP-C mutant lung tissue (immune)
GSM8417639 injured region 1_young ozoneSP-C mutant lung tissue (non immune)
Relations
BioProject PRJNA1139846

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Supplementary file Size Download File type/resource
GSE273048_332_333_5_WTA.jpg.gz 110.0 Mb (ftp)(http) JPG
GSE273048_332_333_LabWorksheet.txt.gz 1.3 Kb (ftp)(http) TXT
GSE273048_89_91_5_WTA.png.gz 72.1 Mb (ftp)(http) PNG
GSE273048_89_91_LabWorksheet.txt.gz 1.2 Kb (ftp)(http) TXT
GSE273048_Mm_R_NGS_WTA_v1.0.pkc.gz 1.8 Mb (ftp)(http) PKC
GSE273048_RAW.tar 2.2 Mb (http)(custom) TAR (of DCC)
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