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Series GSE27300 Query DataSets for GSE27300
Status Public on Feb 10, 2012
Title Estrogen-independent genomic ER binding analysis
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Hormone-independent breast cancer cells (MCF-7/LTED and HCC-1428/LTED) were analyzed by ChIP-seq for estrogen receptor a to identify ER-DNA binding events that occur in the absence of estrogens.
Overall design MCF-7/LTED and HCC-1428/LTED cells were maintained in IMEM + 10% DCC-FBS (dextran-charcoal-treated serum). ChIP for ERa was performed, following by high-throughput DNA sequencing.
Contributor(s) Miller TW, Arteaga CL
Citation(s) 22049316
Submission date Feb 14, 2011
Last update date May 15, 2019
Contact name Todd W Miller
Organization name Vanderbilt Univ
Department Medicine
Street address 2220 Pierce Ave, 777 PRB
City Nashville
State/province TN
ZIP/Postal code 37174
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (2)
GSM675073 MCF-7,LTED_ER-ChIP-seq
GSM675074 HCC-1428,LTED_ER-ChIP-seq
This SubSeries is part of SuperSeries:
GSE37955 ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer
SRA SRP005707
BioProject PRJNA137117

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Supplementary file Size Download File type/resource
GSE27300_RAW.tar 160.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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