|
| Status |
Public on Feb 10, 2012 |
| Title |
Estrogen-independent genomic ER binding analysis |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Hormone-independent breast cancer cells (MCF-7/LTED and HCC-1428/LTED) were analyzed by ChIP-seq for estrogen receptor a to identify ER-DNA binding events that occur in the absence of estrogens.
|
| |
|
| Overall design |
MCF-7/LTED and HCC-1428/LTED cells were maintained in IMEM + 10% DCC-FBS (dextran-charcoal-treated serum). ChIP for ERa was performed, following by high-throughput DNA sequencing.
|
| |
|
| Contributor(s) |
Miller TW, Arteaga CL |
| Citation(s) |
22049316 |
| |
| Submission date |
Feb 14, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Todd W Miller |
| E-mail(s) |
todd.miller@vanderbilt.edu
|
| Organization name |
Vanderbilt Univ
|
| Department |
Medicine
|
| Street address |
2220 Pierce Ave, 777 PRB
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37174 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE37955 |
ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer |
|
| Relations |
| SRA |
SRP005707 |
| BioProject |
PRJNA137117 |
