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| Status |
Public on Sep 17, 2024 |
| Title |
Mid-Lobular Hepatocytes Exhibit Transient Proliferation Pause and Stress Response through Atf4-Ddit3 axis in Early Acute Liver Injury (10x Visium) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Liver zonation remains a critical aspect of understanding its response to acute injury. This study investigates the impact of acetaminophen-induced acute liver injury on zonal heterogeneity during early phases of injury. Through Ki67 staining, we observed a transient pause in proliferation specifically among mid-lobular hepatocytes during the initiation phase. Using spatial transcriptomics, immunostaining, and in vivo assays, we elucidated that mid-lobular hepatocytes upregulate the Atf4-Ddit3 axis, offering temporary protection at the cost of reduced proliferation mediated by Btg2. Our findings underscore the unique zonal metabolism of acetaminophen as a determinant of differential tissue responses across lobular regions. This study highlights how distinct liver zones exhibit varied responses during the early stages of acute injury, with mid-lobular hepatocytes showing an integrated stress response characterized by protective mechanisms that temporarily suppress proliferation.
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| Overall design |
To understand the temporal spatial response to acute APAP injury, liver sections from wild-type mice treated with APAP for 0 h to 72 h were subjected to spatial transcriptomics (ST) using the Visium (10 × Genomics) platform.
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| Contributor(s) |
Zhu Y, Deng C, Chen B, He J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 18, 2024 |
| Last update date |
Sep 18, 2024 |
| Contact name |
Yaying Zhu |
| E-mail(s) |
zhuyaying@stu.ynu.edu.cn
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| Phone |
15624985711
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| Organization name |
yunnan university
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| Street address |
Chenggong district of yunnan university in kunming, yunnan province
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| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE272566 |
Mid-Lobular Hepatocytes Exhibit Transient Proliferation Pause and Stress Response through Atf4-Ddit3 axis in Early Acute Liver Injury |
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| Relations |
| BioProject |
PRJNA1137353 |
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