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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 31, 2024 |
| Title |
An essential role for Cmtr2 in mammalian embryonic development [scRNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CMTR2 is an mRNA cap methyltransferase with poorly understood physiological functions. It catalyzes 2'-O-ribose methylation of the second transcribed nucleotide of mRNAs, potentially serving to mark RNAs as “self” to evade the cellular innate immune response. Here we analyze the consequences of Cmtr2 deficiency in mice. We discover that constitutive deletion of Cmtr2 results in mouse embryos that die during mid-gestation, exhibiting defects in embryo size, placental malformation and yolk sac vascularization. Endothelial cell deletion of Cmtr2 in mice results in vascular and hematopoietic defects, and perinatal lethality. Detailed characterization of the constitutive Cmtr2 KO phenotype shows an activation of the p53 pathway and decreased proliferation, but no evidence of interferon pathway activation. In summary, our study reveals the essential roles of Cmtr2 in mammalian cells beyond its immunoregulatory function.
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| Overall design |
To identify potential targets of Cmtr2, we performed and analyzed single-nucleus RNA sequencing (snRNA-seq) from four Cmtr2+/+ and five Cmtr2-/- E9.5 embryos from 5 different litters.
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| Contributor(s) |
Yermalovich AV, Mohsenin Z, Cowdin M, Giotti B, Gupta A, Feng A, Golomb L, Wheeler DB, Xu K, Tsankov A, Cleaver O, Meyerson M |
| Citation(s) |
39094818 |
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| Submission date |
Jun 27, 2024 |
| Last update date |
Oct 31, 2024 |
| Contact name |
Alena Yermalovich |
| E-mail(s) |
avy424@mail.harvard.edu
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| Organization name |
Dana-Farber Cancer Institute
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| Street address |
450 Brookline Avenue
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| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| GSM8366422 |
Embryo_19, E9.5, KO |
| GSM8366423 |
Embryo_4, E9.5, KO |
| GSM8366424 |
Embryo_2, E9.5, WT |
| GSM8366425 |
Embryo_8, E9.5, WT |
| GSM8366426 |
Embryo_9, E9.5, WT |
| GSM8366427 |
Embryo_13, E9.5, WT |
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| Relations |
| BioProject |
PRJNA1129128 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270969_RAW.tar |
413.9 Mb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
| Raw data are available in SRA |
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