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Series GSE2706 Query DataSets for GSE2706
Status Public on Sep 01, 2005
Title TLR agonists on human dendritic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Toll like receptors (TLRs) sense microbial products and initiate adaptive immune responses by activating dendritic cells (DCs). Since pathogens may contain several agonists we asked whether different TLRs may synergize in DC activation. We report that in human and mouse DC TLR3 or TLR4 potently synergize with TLR7, TLR8 or TLR9 in the induction of selected cytokine genes. Upon synergistic stimulation, IL-12, IL-23 and Delta-4 are induced at levels 50-100 fold higher than those induced by optimal concentrations of single agonists, leading to enhanced and sustained TH1 polarizing capacity. Using microarray analysis we show that only 1.5% of the transcripts induced by single TLR agonists are synergistically regulated by combinations of TLR4 and TLR8 agonists.. These results identify a combinatorial code by which DCs discriminate pathogens and provide (suggest) a rationale to design adjuvants for TH1 responses.
Series_overall_design: 3 untreated, 3 treated with LPS at 2h, 3 treated with LPS at 8h, 3 treated with R848 at 2h, 3 treated with R848 at 8h, 3 treated with LPS + R848 at 2h, 3 treated with LPS + R848 at 8h
Keywords: other
Contributor(s) Napolitani G, Rinaldi A, Bertoni F, Sallusto F, Lanzavecchia A
Citation(s) 15995707
Submission date May 24, 2005
Last update date Jan 08, 2019
Contact name Francesco Bertoni
Phone 0041918200367
Fax 0041918200397
Organization name Institute of Southern Switzerland
Department Experimental Oncology
Lab Functional Genomics
Street address Via Vela 6
City Bellinzona
State/province Ticino
ZIP/Postal code 6500
Country Switzerland
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (21)
GSM52340 LPS 2h 1
GSM52341 R848 2h 1
GSM52342 LPS+R848 2h 1
BioProject PRJNA92355

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