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Series GSE27045 Query DataSets for GSE27045
Status Public on Dec 31, 2011
Title Expression data from macrophages isolated from lupus NZB/W mouse kidneys prenephritic, nephritic or after complete remission.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Renal infiltration with mononuclear cells is associated with poor prognosis in SLE. A renal macrophage/dendritic cell signature is associated with onset of nephritis in NZB/W mice and immune modulating therapies can reverse this signature and the associated renal damage despite ongoing immune complex deposition. Our findings suggest that mononuclear phagocytes with an aberrant activation profile contribute to tissue damage in lupus nephritis by mediating both local inflammation and excessive tissue remodeling.
We used microarrays to analyze the gene expression of renal isolated macrophages at early stage of lupus (young) during lupus nephritis (sick) and after induction of remission (Rem)
Overall design NZB/W F4/80hi mouse cells were isolated using flow cytometry; RNA from cells (young, sick and after complete remission) was extracted and processed for hybridization on Affymetrix microarrays.
Contributor(s) Bethunaickan R, Berthier CC, Ramanujam M, Zhang W, Bottinger E, Ivashkiv L, Kretzler M, Davidson A
Citation(s) 21411733
Submission date Feb 03, 2011
Last update date Aug 20, 2013
Contact name Celine C Berthier
Organization name University of Michigan
Street address 1150 W. Medical Center Dr, MSRB2
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
Platforms (1)
GPL7546 Affymetrix GeneChip Mouse Genome 430 2.0 Array [CDF: Mm_ENTREZG_10]
Samples (17)
GSM667532 Young Mac 1
GSM667533 Young Mac 2
GSM667534 Young Mac 3
BioProject PRJNA137639

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Supplementary file Size Download File type/resource
GSE27045_RAW.tar 110.5 Mb (http)(custom) TAR (of CEL)
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Raw data provided as supplementary file
Processed data included within Sample table

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