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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 26, 2011 |
| Title |
Expression data from pancreatic islets from Men1flf RIP-Cre mice, Rbp2flf RIP-Cre mice, Men1flf Rbp2flf RIP-Cre mice and matched control. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The menin tumor suppressor protein (Men1) is deficient in many endocrine tumors and forms an active complex with MLL family histone methyltransferases. This Men1 complex promotes histone H3 lysine 4 trimethylation at target loci including homeobox genes and cyclin-dependent kinase inhibitor genes. The loss of Men1 may be tumorigenic because it leads to decreased histone H3 lysine 4 trimethylation resulting in expressional changes of specific genes.
Reversing tumorigenesis induced by a Men1 deficiency might be achieved by inhibition of histone H3 lysine 4 demethylase Rbp2 (Kdm5a). To this end, pancreatic islets from Men1f|f, Rbp2f|f and Men1f|f Rbp2f|f mice were expression profiled to determine what transcriptional changes induced by a Men1 deficiency are reversed by the loss of Rpb2.
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| Overall design |
Pancreatic islets were isolated from Men1flf RIP-Cre mice, Rbp2flf RIP-Cre mice, Men1flf Rbp2flf RIP-Cre mice and two month old matched control mice. Total mRNA was extracted from islets and expression profiled on microarrays.
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| Contributor(s) |
Lin W, Cherniack A, Meyerson M |
| Citation(s) |
21788502 |
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| Submission date |
Jan 31, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Andrew Cherniack |
| E-mail(s) |
achernia@broadinstitute.org
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| Organization name |
Broad Institute
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| Street address |
301
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| City |
Binney St |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (17)
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| GSM664140 |
wild type pancreatic islet repeat 1 |
| GSM664141 |
wild type pancreatic islet repeat 2 |
| GSM664142 |
wild type pancreatic islet repeat 3 |
| GSM664143 |
RIP-Cre pancreatic islet repeat 1 |
| GSM664144 |
RIP-Cre pancreatic islet repeat 2 |
| GSM664145 |
RIP-Cre pancreatic islet repeat 3 |
| GSM664146 |
Men1flf, RIP-Cre pancreatic islet repeat 1 |
| GSM664147 |
Men1flf, RIP-Cre pancreatic islet repeat 2 |
| GSM664148 |
Men1flf, RIP-Cre pancreatic islet repeat 3 |
| GSM664149 |
Rbp2flf, RIP-Cre pancreatic islet repeat 1 |
| GSM664150 |
Rbp2flf, RIP-Cre pancreatic islet repeat 2 |
| GSM664151 |
Rbp2flf, RIP-Cre pancreatic islet repeat 3 |
| GSM664152 |
Rbp2flf, RIP-Cre pancreatic islet repeat 4 |
| GSM664153 |
Men1flf, Rbp2flf, RIP-Cre pancreatic islet repeat 1 |
| GSM664154 |
Men1flf, Rbp2flf, RIP-Cre pancreatic islet repeat 2 |
| GSM664155 |
Men1flf, Rbp2flf, RIP-Cre pancreatic islet repeat 3 |
| GSM664156 |
Men1flf, Rbp2flf, RIP-Cre pancreatic islet repeat 4 |
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| Relations |
| BioProject |
PRJNA135923 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26978_RAW.tar |
76.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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