|
| Status |
Public on Mar 31, 2011 |
| Title |
Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr15 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
Methylation profiling by genome tiling array
|
| Summary |
Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications.
Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15.
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| |
|
| Overall design |
ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr15 in the maternal allele and paternal allele in Patdup.dist7=MatDup.dist15 and Patdup.dist7=MatDupdis15 MEFs, respectively, using Nimblegen tiling arrays for chr15.
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| |
|
| Contributor(s) |
Singh P, Wu X, Szabo PE |
| Citation(s) |
21321082 |
| |
| Submission date |
Jan 26, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Xiwei Wu |
| E-mail(s) |
xwu@coh.org
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Computational and Quantitative Medicine
|
| Street address |
1500 E. Duarte Rd.
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11659 |
Mouse distal chr15 tiling array |
|
| Samples (10)
|
| GSM662285 |
CTCFin the maternal allele along distal chr15 |
| GSM662286 |
CTCFin the paternal allele along distal chr15 |
| GSM662287 |
H3K9ac in the maternal allele along distal chr15 |
| GSM662288 |
H3K9ac in the paternal allele along distal chr15 |
| GSM662289 |
H3K4me3 in the maternal allele along distal chr15 |
| GSM662290 |
H3K4me3 in the paternal allele along distal chr15 |
| GSM662291 |
H3K9me3 in the maternal allele along distal chr15 |
| GSM662292 |
H3K9me3 in the paternal allele along distal chr15 |
| GSM662293 |
DNA methylation in the maternal allele along distal chr15 |
| GSM662294 |
DNA methylation in the paternal allele along distal chr15 |
|
| This SubSeries is part of SuperSeries: |
| GSE26947 |
Chromosome wide analysis of parental allele specific chromatin and DNA methylation in mouse |
|
| Relations |
| BioProject |
PRJNA141997 |
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