|
| Status |
Public on May 28, 2024 |
| Title |
Single-cell RNA sequencing of antigen specific CD4+ T cells induced by high vs low doses of MHC-II restricted neoantigens |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we report the impact of the dose of the MHC-II restricted neoantigens on the phenotypic and the effector function of tumor-specific CD4 T cells. Whereas vaccines containing low doses of MHC-II-restricted neoantigen (LDVax) generated helper CD4 T cells that promote the proliferation and the cytotoxic functions of CD8 T cells, vaccines containing high doses of MHC-II restricted neoantigen (HDVax) elicited type 1 regulatory T cells that suppress the antitumor immunity.
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| |
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| Overall design |
T3 tumor-bearing 129S6 male mice were vaccinated on day 6 post-tumor implantation, and 7 days later, tumors were harvested and processed into single-cell suspension. CD4+ T cells were then enriched using positive selection beads and then FACS sorted to ensure high purity (>95%).
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| |
|
| Contributor(s) |
Schreiber R, Sultan H |
| Citation(s) |
39048822 |
| |
| Submission date |
May 24, 2024 |
| Last update date |
Aug 11, 2024 |
| Contact name |
Vladimir Sukhov |
| E-mail(s) |
sukhov@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Immunology
|
| Street address |
660. S Euclid Ave.
|
| City |
St. Louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (9)
|
| GSM8289316 |
PBS (PBS treated CD4+ T cells from T3 TILs) |
| GSM8289317 |
PBS (PBS treated CD4+ T cells from T3 TILs) TCR |
| GSM8289318 |
PBS (PBS treated CD4+ T cells from T3 TILs) FBC |
| GSM8289319 |
LD (LDVax treated CD4+ T cells from T3 TILs) |
| GSM8289320 |
LD (LDVax treated CD4+ T cells from T3 TILs) TCR |
| GSM8289321 |
LD (LDVax treated CD4+ T cells from T3 TILs) FBC |
| GSM8289322 |
HD (HDVax treated CD4+ T cells from T3 TILs) |
| GSM8289323 |
HD (HDVax treated CD4+ T cells from T3 TILs) TCR |
| GSM8289324 |
HD (HDVax treated CD4+ T cells from T3 TILs) FBC |
|
| This SubSeries is part of SuperSeries: |
| GSE268302 |
RNA sequencing of antigen specific CD4+ T cells induced by high vs low doses of MHC-II restricted neoantigens |
|
| Relations |
| BioProject |
PRJNA1116136 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE268301_HD_barcodes.tsv.gz |
56.3 Kb |
(ftp)(http) |
TSV |
| GSE268301_HD_features.tsv.gz |
284.2 Kb |
(ftp)(http) |
TSV |
| GSE268301_HD_filtered_contig_annotations.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE268301_HD_matrix.mtx.gz |
52.7 Mb |
(ftp)(http) |
MTX |
| GSE268301_LD_barcodes.tsv.gz |
24.1 Kb |
(ftp)(http) |
TSV |
| GSE268301_LD_features.tsv.gz |
284.2 Kb |
(ftp)(http) |
TSV |
| GSE268301_LD_filtered_contig_annotations.csv.gz |
547.5 Kb |
(ftp)(http) |
CSV |
| GSE268301_LD_matrix.mtx.gz |
27.3 Mb |
(ftp)(http) |
MTX |
| GSE268301_PBS_barcodes.tsv.gz |
50.1 Kb |
(ftp)(http) |
TSV |
| GSE268301_PBS_features.tsv.gz |
284.2 Kb |
(ftp)(http) |
TSV |
| GSE268301_PBS_filtered_contig_annotations.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE268301_PBS_matrix.mtx.gz |
57.2 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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