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| Status |
Public on May 28, 2024 |
| Title |
Gene expresion profile of different clusters of antigen specific CD4+ T cells induced by vaccines containing high doses of MHC-II restricted neoantigens |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
CD4+ T cells induced by vaccines containing high doses of mItgb1 SLP were sorted into 3 different clusters based on the expression of CD25, CD200 and CD39. We found that cluster-3 cells (CD4+ CD25- and CD39+) express high levels of GZMB, Ccl5, Lilirb4 and Sema4a. The gene signature of cluster-3 matched those of mice and human Tr1.
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| Overall design |
T3 tumor-bearing 129S6 male mice were vaccinated on day 6 post-tumor implantation, and 7 days later, tumors were harvested and processed into single-cell suspension. Antigen specific CD4+ T cells were then enriched using positive selection beads and then FACS sorted into 3 differtent clusters as listed.
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| Contributor(s) |
Schreiber R, Sultan H |
| Citation(s) |
39048822 |
| |
| Submission date |
May 24, 2024 |
| Last update date |
Aug 11, 2024 |
| Contact name |
Vladimir Sukhov |
| E-mail(s) |
sukhov@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Immunology
|
| Street address |
660. S Euclid Ave.
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| City |
St. Louis |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE268302 |
RNA sequencing of antigen specific CD4+ T cells induced by high vs low doses of MHC-II restricted neoantigens |
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| Relations |
| BioProject |
PRJNA1116135 |
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